PA6 Stromal Cell Co-Culture Enhances SH-SY5Y and VSC4.1 Neuroblastoma Differentiation to Mature Phenotypes

Neuroblastoma cell lines such as SH-SY5Y have been used for modelling neurodegenerative diseases and for studying basic mechanisms in neuroscience. Since neuroblastoma cells proliferate and generally do not express markers of mature or functional neurons, we exploited a co-culture system with the st...

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Bibliographic Details
Published in:PloS one 2016-07, Vol.11 (7), p.e0159051-e0159051
Main Authors: Ferguson, Ross, Subramanian, Vasanta
Format: Article
Language:English
Subjects:
Amyotrophic lateral sclerosis
Antigens, Differentiation - metabolism
Axonogenesis
Biochemistry
Biology and Life Sciences
Biotechnology
Cell culture
Cell cycle
Cell differentiation
Cell Differentiation - drug effects
Cell Line, Tumor
Cell lines
Coculture Techniques
Differentiation
Dopamine
Embryos
Genetic aspects
Growth factors
Humans
Markers
Medicine and Health Sciences
Motor neuron diseases
Motor neurone disease
Nervous system
Nervous system diseases
Neurites - metabolism
Neurites - pathology
Neuroblastoma
Neuroblastoma - metabolism
Neuroblastoma - pathology
Neuroblastoma cells
Neuroblasts
Neurodegenerative diseases
Neurological diseases
Neurons
Phenotypes
Physiological aspects
Properties
Proteins
Retinoic acid
Stem cells
Stromal Cells - metabolism
Stromal Cells - pathology
Tretinoin - pharmacology
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Summary:Neuroblastoma cell lines such as SH-SY5Y have been used for modelling neurodegenerative diseases and for studying basic mechanisms in neuroscience. Since neuroblastoma cells proliferate and generally do not express markers of mature or functional neurons, we exploited a co-culture system with the stromal cell line PA6 to better induce differentiation to a more physiologically relevant status. We found that co-culture of the neuroblastoma cell lines in the presence of neural inducers such retinoic acid was able to generate a high proportion of quiescent neurons with very long neurites expressing differentiation markers. The co-culture system additionally cuts short the time taken to produce a more mature phenotype. We also show the application of this system to study proteins implicated in motor neuron disease.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0159051