Whole Genome Sequencing Allows Better Understanding of the Evolutionary History of Leptospira interrogans Serovar Hardjo

The genome of a laboratory-adapted strain of Leptospira interrogans serovar Hardjo was sequenced and analyzed. Comparison of the sequenced genome with that recently published for a field isolate of the same serovar revealed relatively high sequence conservation at the nucleotide level, despite the d...

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Bibliographic Details
Published in:PloS one 2016-07, Vol.11 (7), p.e0159387-e0159387
Main Authors: Llanes, Alejandro, Restrepo, Carlos Mario, Rajeev, Sreekumari
Format: Article
Language:English
Subjects:
Adaptation
Annotations
Base Sequence
Bioinformatics
Biological Evolution
Biology and Life Sciences
Chromosomes
Computer and Information Sciences
Conservation
Conserved sequence
Deoxyribonucleic acid
DNA
Drug resistance
Gene sequencing
Gene transfer
Genes
Genome, Bacterial
Genomes
Genomics
Laboratories
Leptospira
Leptospira interrogans
Leptospira interrogans - classification
Leptospira interrogans - genetics
Likelihood Functions
Loci
Medicine and Health Sciences
Molecular Sequence Annotation
Next-generation sequencing
Phylogenetics
Phylogeny
Proteins
Research and Analysis Methods
Sequence Analysis, DNA - methods
Serogroup
Veterinary medicine
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Summary:The genome of a laboratory-adapted strain of Leptospira interrogans serovar Hardjo was sequenced and analyzed. Comparison of the sequenced genome with that recently published for a field isolate of the same serovar revealed relatively high sequence conservation at the nucleotide level, despite the different biological background of both samples. Conversely, comparison of both serovar Hardjo genomes with those of L. borgpetersenii serovar Hardjo showed extensive differences between the corresponding chromosomes, except for the region occupied by their rfb loci. Additionally, comparison of the serovar Hardjo genomes with those of different L. interrogans serovars allowed us to detect several genomic features that may confer an adaptive advantage to L. interrogans serovar Hardjo, including a possible integrated plasmid and an additional copy of a cluster encoding a membrane transport system known to be involved in drug resistance. A phylogenomic strategy was used to better understand the evolutionary position of the Hardjo serovar among L. interrogans serovars and other Leptospira species. The proposed phylogeny supports the hypothesis that the presence of similar rfb loci in two different species may be the result of a lateral gene transfer event.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0159387