Contrasting Potato Foliage and Tuber Defense Mechanisms against the Late Blight Pathogen Phytophthora infestans

The late blight pathogen Phytophthora infestans can attack both potato foliage and tubers. When inoculated with P. infestans, foliage of nontransformed 'Russet Burbank' (WT) develops late blight disease while that of transgenic 'Russet Burbank' line SP2211 (+RB) does not. We comp...

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Bibliographic Details
Published in:PloS one 2016-07, Vol.11 (7), p.e0159969-e0159969
Main Authors: Gao, Liangliang, Bradeen, James M
Format: Article
Language:English
Subjects:
Analogies
Bins
Biology and Life Sciences
Blight
Cell walls
Cloning
Cluster Analysis
Comparative analysis
Computational Biology - methods
Defense mechanisms
Development and progression
Disease resistance (Plants)
Disease Resistance - genetics
Diseases and pests
Foliage
Gene dosage
Gene expression
Gene Expression Profiling
Gene Ontology
Genes
Genetic aspects
Genome, Plant
Genomes
Genomics
Genotype
High-Throughput Nucleotide Sequencing
Host-Parasite Interactions - genetics
Inoculation
Kinases
Late blight
Leaves
Ontology
Pathogens
Phenotype
Physical Sciences
Physiological aspects
Phytophthora diseases
Phytophthora infestans
Plant Diseases - genetics
Plant Diseases - parasitology
Plant pathology
Plant Tubers - genetics
Plant Tubers - parasitology
Potatoes
Research and analysis methods
Ribonucleic acid
RNA
Solanum tuberosum
Solanum tuberosum - genetics
Solanum tuberosum - parasitology
Studies
Transcription
Transcriptome
Tubers
Vegetables
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Summary:The late blight pathogen Phytophthora infestans can attack both potato foliage and tubers. When inoculated with P. infestans, foliage of nontransformed 'Russet Burbank' (WT) develops late blight disease while that of transgenic 'Russet Burbank' line SP2211 (+RB) does not. We compared the foliar transcriptome responses of these two lines to P. infestans inoculation using an RNA-seq approach. A total of 515 million paired end RNA-seq reads were generated, representing the transcription of 29,970 genes. We also compared the differences and similarities of defense mechanisms against P. infestans in potato foliage and tubers. Differentially expressed genes, gene groups and ontology bins were identified to show similarities and differences in foliage and tuber defense mechanisms. Our results suggest that R gene dosage and shared biochemical pathways (such as ethylene and stress bins) contribute to RB-mediated incompatible potato-P. infestans interactions in both the foliage and tubers. Certain ontology bins such as cell wall and lipid metabolisms are potentially organ-specific.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0159969