Estimation of Tau and Phosphorylated Tau181 in Serum of Alzheimer's Disease and Mild Cognitive Impairment Patients

The elevated level of cerebrospinal fluid (CSF) Tau and phosphorylated Tau181 (p-Tau181) proteins are well established hallmarks of Alzheimer's disease (AD). Elevated level of p-Tau181 can differentiate AD from other neurodegenerative disease. However, the expression level of these proteins in...

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Bibliographic Details
Published in:PloS one 2016-07, Vol.11 (7), p.e0159099-e0159099
Main Authors: Shekhar, Shashank, Kumar, Rahul, Rai, Nitish, Kumar, Vijay, Singh, Kusum, Upadhyay, Ashish Datt, Tripathi, Manjari, Dwivedi, Sadanand, Dey, Aparajit B, Dey, Sharmistha
Format: Article
Language:English
Subjects:
Activities of daily living
Aged
Alzheimer Disease - blood
Alzheimer Disease - diagnosis
Alzheimer's disease
Alzheimers disease
Antibodies
Biology and Life Sciences
Biomarkers
Biophysics
Case-Control Studies
Cerebrospinal fluid
Cognitive ability
Cognitive Dysfunction - blood
Cognitive Dysfunction - diagnosis
Dementia
Ethics
Female
Geriatrics
Humans
Impairment
Male
Medicine and Health Sciences
Memory
Middle Aged
Neurodegenerative diseases
Neurology
Older people
Patients
People and Places
Phosphoproteins - blood
Phosphorylation
Plasma
Proteins
Risk Factors
ROC Curve
Statistical analysis
Statistical methods
Surface plasmon resonance
Tau protein
tau Proteins - blood
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Summary:The elevated level of cerebrospinal fluid (CSF) Tau and phosphorylated Tau181 (p-Tau181) proteins are well established hallmarks of Alzheimer's disease (AD). Elevated level of p-Tau181 can differentiate AD from other neurodegenerative disease. However, the expression level of these proteins in serum of AD patient is not well set up. This study sought to evaluate the level of Tau and p-Tau181 in serum of AD, and mild cognitive impairment (MCI) patients for an alternative approach to establish protein-based markers by convenient way. Blood samples were collected from 39 AD patients, 37 MCI patients and 37 elderly individuals as controls. The levels of Tau and p-Tau181 in the serum of the different groups were measured by label free real time Surface Plasmon Resonance technology by using specific antibodies, and were further confirmed by the conventional western blot method. An appropriate statistical analysis, including Receiver Operating Characteristic (ROC), was performed. The concentrations of serum Tau and p-Tau181 were significantly higher (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0159099