Comparison of miRNAs and Their Targets in Seed Development between Two Maize Inbred Lines by High-Throughput Sequencing and Degradome Analysis

MicroRNAs (miRNAs) play an important role in plant growth, development, and response to environment. For identifying and comparing miRNAs and their targets in seed development between two maize inbred lines (i.e. PH6WC and PH4CV), two sRNAs and two degradome libraries were constructed. Through high-...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2016-07, Vol.11 (7), p.e0159810
Main Authors: Wu, Feng-Yao, Tang, Cheng-Yi, Guo, Yu-Min, Yang, Min-Kai, Yang, Rong-Wu, Lu, Gui-Hua, Yang, Yong-Hua
Format: Article
Language:English
Subjects:
Analysis
Arabidopsis
Arabidopsis thaliana
Biology and life sciences
Biotechnology
Conserved Sequence
Corn
Degradation
Flowering
Flowers & plants
Gene expression
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genomics
Identification
Inbreeding
Laboratories
Libraries
Life sciences
MicroRNA
MicroRNAs
MicroRNAs - genetics
miRNA
Molecular biology
Morphology
Next-generation sequencing
Ontology
Pharmaceuticals
Plant development
Plant growth
Research and Analysis Methods
RNA, Plant - genetics
Seeds
Seeds - genetics
Seeds - growth & development
Zea mays
Zea mays - genetics
Zea mays - growth & development
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:MicroRNAs (miRNAs) play an important role in plant growth, development, and response to environment. For identifying and comparing miRNAs and their targets in seed development between two maize inbred lines (i.e. PH6WC and PH4CV), two sRNAs and two degradome libraries were constructed. Through high-throughput sequencing and miRNA identification, 55 conserved and 24 novel unique miRNA sequences were identified in two sRNA libraries; moreover, through degradome sequencing and analysis, 137 target transcripts corresponding to 38 unique miRNA sequences were identified in two degradome libraries. Subsequently, 16 significantly differentially expressed miRNA sequences were verified by qRT-PCR, in which 9 verified sequences obviously target 30 transcripts mainly involved with regulation in flowering and development in embryo. Therefore, the results suggested that some miRNAs (e.g. miR156, miR171, miR396 and miR444) related reproductive development might differentially express in seed development between the PH6WC and PH4CV maize inbred lines in this present study.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0159810