Transcriptome Analysis of Storage Roots and Fibrous Roots of the Traditional Medicinal Herb Callerya speciosa (Champ.) ScHot

• Published in: PloS one 2016-08, Vol.11 (8), p.e0160338-e0160338
• Main Authors: Xu, Li, Wang, Jiabin, Lei, Ming, Li, Li, Fu, Yunliu, Wang, Zhunian, Ao, Mengfei, Li, Zhiying
• Format: Article
• Language: English
• Subjects: Agriculture; Analysis; Biology; Biology and Life Sciences; Biosynthesis; Breeding; Callerya speciosa; Cell division; Cell walls; DNA sequencing; Fabaceae; Fabaceae - genetics; Fabaceae - metabolism; Flavonoids; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene families; Gene Ontology; Gene sequencing; Genes; Genes, Plant; Genomes; Genomics; Germplasm; Herbal medicine; High-Throughput Nucleotide Sequencing; Kinases; Laboratories; Loosening; Medicinal plants; Metabolites; Physical Sciences; Physiological aspects; Plant breeding; Plant metabolites; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - genetics; Plant Roots - metabolism; Plant sciences; Plants, Medicinal - genetics; Plants, Medicinal - metabolism; Potatoes; Proteins; Research and analysis methods; Root development; Roots; Secondary metabolites; Sequence Analysis, DNA; Signaling; Starch; Storage; Sucrose; Sugar; Transcription (Genetics); Transcription factors; Transcriptome; Trends
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0160338

Callerya speciosa (Champ.) ScHot is a woody perennial plant in Fabaceae, the roots of which are used medicinally. The storage roots of C. speciosa are derived from fibrous roots, but not all fibrous roots can develop into storage roots. To detect key genes involved in storage roots formation, we performed Illumina sequencing of the C. speciosa storage roots and fibrous roots. De novo assembly resulted in 161,926 unigenes, which were subsequently annotated by BLAST, GO and KEGG analyses. After expression profiling, 4538 differentially expressed genes were identified. The KEGG pathway enrichment analysis revealed changes in the biosynthesis of cytokinin, phenylpropanoid, starch, sucrose, flavone and other secondary metabolites. Transcription factor-related differentially expressed genes (DEGs) were also identified, including such gene families as GRAS, COL, MIKC, ERF, LBD, and NAC. The DEGs related to light signaling, starch, sugar, photohormones and cell wall-loosening might be involved in the formation of storage roots. This study provides the first transcriptome profiling of C. speciosa roots, data that will facilitate future research of root development and metabolites with medicinal value as well as the breeding of C. speciosa.