The Grapevine Uncharacterized Intrinsic Protein 1 (VvXIP1) Is Regulated by Drought Stress and Transports Glycerol, Hydrogen Peroxide, Heavy Metals but Not Water

• Published in: PloS one 2016-08, Vol.11 (8), p.e0160976
• Main Authors: Noronha, Henrique, Araújo, Diogo, Conde, Carlos, Martins, Ana P, Soveral, Graça, Chaumont, François, Delrot, Serge, Gerós, Hernâni
• Format: Article
• Language: English
• Subjects: Analysis; Aquaporins; Arabidopsis; Arsenic; Biodiversity; Biological Transport; Biology; Biology and Life Sciences; Boric acid; Coding; Copper; Drought; Droughts; Endoplasmic reticulum; Flowers & plants; Fungi; Gene Expression Regulation, Plant; Genomes; Glycerol; Glycerol - metabolism; Health aspects; Heavy metals; Homeostasis; Hydrogen; Hydrogen ion concentration; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Hydrogen storage; Impact tests; Imposition; Influence; Intracellular Space - metabolism; Irrigation; Leaves; Life Sciences; Localization; Membrane vesicles; Metals, Heavy - metabolism; Nickel; Nicotiana benthamiana; Osmotic stress; Pharmaceutical sciences; Phylogenetics; Physcomitrella patens; Physical sciences; Plant Proteins - genetics; Plant Proteins - metabolism; Protein Transport; Proteins; Spectrometry; Stress, Physiological; Transcription, Genetic; Transformation; Transport; Vegetal Biology; Vines; Vitaceae; Vitis - cytology; Vitis - genetics; Vitis - metabolism; Vitis - physiology; Vitis vinifera; Water - metabolism; Yeast; Yeasts
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0160976

A MIP (Major Intrinsic Protein) subfamily called Uncharacterized Intrinsic Proteins (XIP) was recently described in several fungi and eudicot plants. In this work, we cloned a XIP from grapevine, VvXIP1, and agrobacterium-mediated transformation studies in Nicotiana benthamiana revealed that the encoded aquaporin shows a preferential localization at the endoplasmic reticulum membrane. Stopped-flow spectrometry in vesicles from the aqy-null yeast strain YSH1172 overexpressing VvXIP1 showed that VvXIP1 is unable to transport water but is permeable to glycerol. Functional studies with the ROS sensitive probe CM-H2DCFDA in intact transformed yeasts showed that VvXIP1 is also able to permeate hydrogen peroxide (H2O2). Drop test growth assays showed that besides glycerol and H2O2, VvXIP1 also transports boric acid, copper, arsenic and nickel. Furthermore, we found that VvXIP1 transcripts were abundant in grapevine leaves from field grown plants and strongly repressed after the imposition of severe water-deficit conditions in potted vines. The observed downregulation of VvXIP1 expression in cultured grape cells in response to ABA and salt, together with the increased sensitivity to osmotic stress displayed by the aqy-null yeast overexpressing VvXIP1, corroborates the role of VvXIP1 in osmotic regulation besides its involvement in H2O2 transport and metal homeostasis.