Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities

• Published in: PloS one 2016-08, Vol.11 (8), p.e0160799
• Main Authors: Liu, Min, Jing, Huijuan, Zhang, Jianjun, Che, Gen, Zhou, Meng, Gao, Zheng, Li, Shangshang, Ren, Zhenzhen, Hao, Long, Liu, Yu, Jia, Le
• Format: Article
• Language: English
• Subjects: Aging; Aging (artificial); Aging (Biology); Aging (natural); Aging - drug effects; Agriculture; Agrocybe - chemistry; Animals; Antioxidants; Antioxidants (Nutrients); Antioxidants - isolation & purification; Antioxidants - pharmacology; Arabinose; Bioaccumulation; Biocompatibility; Biology and Life Sciences; Care and treatment; Cholesterol; D-Galactose; Drugs; Engineering research; Experimental design; Food; Free radicals; Functional foods; Functional foods & nutraceuticals; Galactose; Glutathione; Glutathione peroxidase; Glutathione Peroxidase - metabolism; Laboratories; Life sciences; Liver - drug effects; Liver - metabolism; Malonaldehyde; Malondialdehyde; Malondialdehyde - metabolism; Mannose; Mice; Monosaccharides - analysis; Mushrooms; Mycelia; Mycelium - chemistry; Optimization; Organoselenium Compounds - isolation & purification; Organoselenium Compounds - pharmacology; Oxidative stress; Oxygen; Peroxidase; pH effects; Physical Sciences; Physiological aspects; Physiology; Polysaccharides; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Properties; Purification; Reactive oxygen species; Rhamnose; Saccharides; Selenium; Selenium - analysis; Studies; Superoxide dismutase; Superoxide Dismutase - metabolism; Temperature; Urban farming
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0160799

The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities. The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%. The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species. The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing. Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7. These results suggest that MSPS might be suitable for functional foods and natural drugs on preventing the ageing progress induced by toxic chemicals.