The Complete Chloroplast Genome Sequence of a Relict Conifer Glyptostrobus pensilis: Comparative Analysis and Insights into Dynamics of Chloroplast Genome Rearrangement in Cupressophytes and Pinaceae

• Published in: PloS one 2016-08, Vol.11 (8), p.e0161809-e0161809
• Main Authors: Hao, Zhaodong, Cheng, Tielong, Zheng, Renhua, Xu, Haibin, Zhou, Yanwei, Li, Meiping, Lu, Fengjuan, Dong, Yini, Liu, Xin, Chen, Jinhui, Shi, Jisen
• Format: Article
• Language: English
• Subjects: Analysis; Biodiversity; Biological evolution; Biology and Life Sciences; Biotechnology; Chloroplasts; Comparative analysis; Coniferophyta - classification; Coniferophyta - genetics; Conifers; Conservation; Cupressaceae; Cupressaceae - genetics; Deoxyribonucleic acid; DNA; DNA, Plant - chemistry; DNA, Plant - genetics; Earth Sciences; Education; Endangered & extinct species; Endangered species; Evolution; Evolution, Molecular; Forestry; Gene Rearrangement; Genes, Chloroplast - genetics; Genetic aspects; Genetic diversity; Genetic Variation; Genome, Chloroplast - genetics; Genome, Plant - genetics; Genomes; Internet; Inverted repeat; Inverted Repeat Sequences - genetics; Laboratories; Legumes; Molecular biology; Nucleotide sequence; Phylogenetics; Phylogeny; Physiological aspects; Pinaceae; Pinaceae - classification; Pinaceae - genetics; Plastids; Repetitive Sequences, Nucleic Acid - genetics; Research and Analysis Methods; Sequence Analysis, DNA; Simple sequence repeats; Species diversity; Species Specificity; Splitting; Wildlife conservation
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0161809

Glyptostrobus pensilis, belonging to the monotypic genus Glyptostrobus (Family: Cupressaceae), is an ancient conifer that is naturally distributed in low-lying wet areas. Here, we report the complete chloroplast (cp) genome sequence (132,239 bp) of G. pensilis. The G. pensilis cp genome is similar in gene content, organization and genome structure to the sequenced cp genomes from other cupressophytes, especially with respect to the loss of the inverted repeat region A (IRA). Through phylogenetic analysis, we demonstrated that the genus Glyptostrobus is closely related to the genus Cryptomeria, supporting previous findings based on physiological characteristics. Since IRs play an important role in stabilize cp genome and conifer cp genomes lost different IR regions after splitting in two clades (cupressophytes and Pinaceae), we performed cp genome rearrangement analysis and found more extensive cp genome rearrangements among the species of cupressophytes relative to Pinaceae. Additional repeat analysis indicated that cupressophytes cp genomes contained less potential functional repeats, especially in Cupressaceae, compared with Pinaceae. These results suggested that dynamics of cp genome rearrangement in conifers differed since the two clades, Pinaceae and cupressophytes, lost IR copies independently and developed different repeats to complement the residual IRs. In addition, we identified 170 perfect simple sequence repeats that will be useful in future research focusing on the evolution of genetic diversity and conservation of genetic variation for this endangered species in the wild.