Integration of Next Generation Sequencing and EPR Analysis to Uncover Molecular Mechanism Underlying Shell Color Variation in Scallops

• Published in: PloS one 2016-08, Vol.11 (8), p.e0161876
• Main Authors: Sun, Xiujun, Liu, Zhihong, Zhou, Liqing, Wu, Biao, Dong, Yinghui, Yang, Aiguo
• Format: Article
• Language: English
• Subjects: Albinism; Animal coloration; Animal Shells - metabolism; Animals; Aquaculture; Biology and Life Sciences; Bivalvia; Breeding; Chemical synthesis; Color; Coloration; Dermatology; DNA sequencing; Electron paramagnetic resonance; Electron Spin Resonance Spectroscopy - methods; Fishery sciences; Gene expression; Genes; Genetic aspects; Genomics; High-Throughput Nucleotide Sequencing - methods; Melanin; Melanization; Mollusca; Mollusks; Neurosciences; Patinopecten yessoensis; Pectinidae - genetics; Pheomelanin; Physical Sciences; Physiological aspects; Pigmentation - genetics; Pigmentation - physiology; Pigments; Polymorphism; Post-transcription; Proteins; Research and analysis methods; RNA polymerase; RNA, Messenger - genetics; Scallops; Shellfish; Shells; Signal transduction; Studies; Transcription factors; Tyrosinase
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0161876

The Yesso scallop Patinopecten yessoensis displays polymorphism in shell colors, which is of great interest for the scallop industry. To identify genes involved in the shell coloration, in the present study, we investigate the transcriptome differences by Illumina digital gene expression (DGE) analysis in two extreme color phenotypes, Red and White. Illumina sequencing yields a total of 62,715,364 clean sequence reads, and more than 85% reads are mapped into our previously sequenced transcriptome. There are 25 significantly differentially expressed genes between Red and White scallops. EPR (Electron paramagnetic resonance) analysis has identified EPR spectra of pheomelanin and eumelanin in the red shells, but not in the white shells. Compared to the Red scallops, the White scallops have relatively higher mRNA expression in tyrosinase genes, but lower expression in other melanogensis-associated genes. Meantime, the relatively lower tyrosinase protein and decreased tyrosinase activity in White scallops are suggested to be associated with the lack of melanin in the white shells. Our findings highlight the functional roles of melanogensis-associated genes in the melanization process of scallop shells, and shed new lights on the transcriptional and post-transcriptional mechanisms in the regulation of tyrosinase activity during the process of melanin synthesis. The present results will assist our molecular understanding of melanin synthesis underlying shell color polymorphism in scallops, as well as other bivalves, and also help the color-based breeding in shellfish aquaculture.