Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury

Treatment of acute lung injury (ALI) remains an unsolved problem in intensive care medicine. Recruitment of neutrophils into the lungs, regarded as a key mechanism in progression of ALI, depends on signaling between neutrophils and platelets. Consequently we explored the effect of platelet-targeted...

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Published in:PloS one 2016-09, Vol.11 (9), p.e0161218-e0161218
Main Authors: Tilgner, Jessica, von Trotha, Klaus Thilo, Gombert, Alexander, Jacobs, Michael J, Drechsler, Maik, Döring, Yvonne, Soehnlein, Oliver, Grommes, Jochen
Format: Article
Language:English
Subjects:
Acute Lung Injury - chemically induced
Acute Lung Injury - pathology
Acute Lung Injury - prevention & control
Alveoli
Animals
Aspirin
Biology and Life Sciences
Blood platelets
Care and treatment
Cellular signal transduction
Chemokines
Cytometry
Dextran
Dosage and administration
Edema
Endotoxins - toxicity
Flow cytometry
Inhalation
Injection
Injuries
Laboratory animals
Leukocytes (neutrophilic)
Lipopolysaccharides
Lung diseases
Lungs
Male
Medicine and Health Sciences
Mice
Mice, Inbred C57BL
Neutrophils
Neutrophils - pathology
Permeability
Platelets
Prevention
Proteins
Recruitment
Research and Analysis Methods
Respiration
Rodents
Sepsis
Signaling
Tirofiban
Tyrosine - analogs & derivatives
Tyrosine - pharmacology
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cited_by cdi_FETCH-LOGICAL-c655t-d9535f2347abd1ee3a7429f99ad5c5e447fe855e6ef81d51fd9dd0727ed6572a3
cites cdi_FETCH-LOGICAL-c655t-d9535f2347abd1ee3a7429f99ad5c5e447fe855e6ef81d51fd9dd0727ed6572a3
container_end_page e0161218
container_issue 9
container_start_page e0161218
container_title PloS one
container_volume 11
creator Tilgner, Jessica
von Trotha, Klaus Thilo
Gombert, Alexander
Jacobs, Michael J
Drechsler, Maik
Döring, Yvonne
Soehnlein, Oliver
Grommes, Jochen
description Treatment of acute lung injury (ALI) remains an unsolved problem in intensive care medicine. Recruitment of neutrophils into the lungs, regarded as a key mechanism in progression of ALI, depends on signaling between neutrophils and platelets. Consequently we explored the effect of platelet-targeted aspirin and tirofiban treatment in endotoxin induced acute lung injury. C57Bl/6 mice were exposed to aerosolized LPS (500μg/ml) for 30min and treated with Aspirin (100μg/g bodyweight via intraperitoneal injection, 30 min before or 1 hour after LPS inhalation) or Tirofiban (0.5μg/ g bodyweight via tail vein injection 30 min before or 1 hour after LPS inhalation). The count of alveolar, interstitial, and intravascular neutrophils was assessed 4h later by flow cytometry. Lung permeability changes were assessed by FITC-dextran clearance and protein content in the BAL fluid. Aspirin both before and after LPS inhalation reduced neutrophil influx into the lung and lung permeability indicating the protective role of Aspirin in ALI. Tirofiban, however, did not alter neutrophil recruitment after LPS inhalation. Release of platelet-derived chemokines CCL5 and PF4 and neutrophil extracellular traps was reduced by Aspirin but not by Tirofiban. Aspirin, but not Tirofiban reduces neutrophil recruitment and displays protective effects during endotoxin induced lung injury.
doi_str_mv 10.1371/journal.pone.0161218
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1932-6203
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subjects Acute Lung Injury - chemically induced
Acute Lung Injury - pathology
Acute Lung Injury - prevention & control
Alveoli
Animals
Aspirin
Biology and Life Sciences
Blood platelets
Care and treatment
Cellular signal transduction
Chemokines
Cytometry
Dextran
Dosage and administration
Edema
Endotoxins - toxicity
Flow cytometry
Inhalation
Injection
Injuries
Laboratory animals
Leukocytes (neutrophilic)
Lipopolysaccharides
Lung diseases
Lungs
Male
Medicine and Health Sciences
Mice
Mice, Inbred C57BL
Neutrophils
Neutrophils - pathology
Permeability
Platelets
Prevention
Proteins
Recruitment
Research and Analysis Methods
Respiration
Rodents
Sepsis
Signaling
Tirofiban
Tyrosine - analogs & derivatives
Tyrosine - pharmacology
title Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T01%3A12%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Aspirin,%20but%20Not%20Tirofiban%20Displays%20Protective%20Effects%20in%20Endotoxin%20Induced%20Lung%20Injury&rft.jtitle=PloS%20one&rft.au=Tilgner,%20Jessica&rft.date=2016-09-01&rft.volume=11&rft.issue=9&rft.spage=e0161218&rft.epage=e0161218&rft.pages=e0161218-e0161218&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0161218&rft_dat=%3Cgale_plos_%3EA462201325%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c655t-d9535f2347abd1ee3a7429f99ad5c5e447fe855e6ef81d51fd9dd0727ed6572a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1815978439&rft_id=info:pmid/27583400&rft_galeid=A462201325&rfr_iscdi=true