treA Codifies for a Trehalase with Involvement in Xanthomonas citri subsp. citri Pathogenicity

• Published in: PloS one 2016-09, Vol.11 (9), p.e0162886
• Main Authors: Alexandrino, André Vessoni, Goto, Leandro Seiji, Novo-Mansur, Maria Teresa Marques
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial infections; Biochemistry; Biology and Life Sciences; Canker; Chromatography, Gel; Circular Dichroism; Citrus; Citrus - microbiology; Citrus aurantifolia; Citrus fruits; Clonal deletion; Cloning; Codification; Deoxyribonucleic acid; Disease; DNA; E coli; Enzymatic activity; Enzymes; Gene Deletion; Gene expression; Genes, Bacterial; Genomes; Genomics; Glucose; Hydrolysis; Infectivity; Kinetics; Lysates; Medicine and Health Sciences; Molecular biology; Pathogenicity; Pathogens; Physical Sciences; Plant Diseases - microbiology; Protein structure; Proteins; Quaternary; Quaternary structure; Reaction kinetics; Recombinant proteins; Recombinant Proteins - metabolism; Sugar; Trehalase - genetics; Trehalase - metabolism; Trehalose; Virulence; Virulence (Microbiology); Xanthomonas; Xanthomonas - chemistry; Xanthomonas - enzymology; Xanthomonas - genetics; Xanthomonas - pathogenicity; Xanthomonas citri
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0162886

Citrus canker, caused by the bacterium Xanthomonas citri subsp. citri (Xcc), is a severe disease of citrus. Xcc presents broad spectrum of citrus hosts including economically important species whereas X. fuscans subsp. aurantifolii-type C (XauC) causes a milder disease and only infects Citrus aurantifolia. Trehalase catalyzes hydrolysis of the disaccharide trehalose, a sugar that has been reported to be related to Xcc pathogenicity. We expressed the recombinant gene product and assessed Xcc trehalase structural and kinetics data. The recombinant protein presented 42.7% of secondary structures in α-helix and 13% in β-sheets, no quaternary structure in solution, and Michaelis-Menten constant (KM) of 0.077 mM and Vmax 55.308 μMol glucose.min-1.mg protein-1 for trehalose. A Xcc mutant strain (XccΔtreA) was produced by gene deletion from Xcc genome. Enzymatic activity of trehalase was determined in Xcc, XauC and XccΔtreA cellular lysates, showing the highest values for XauC in in vitro infective condition and no activity for XccΔtreA. Finally, leaves of Citrus aurantifolia infected with XccΔtreA showed much more drenching and necrosis than those infected by wild type Xcc. We concluded that trehalase contributes to alleviate bacterial virulence and that inability for trehalose hydrolysis may promote higher Xcc infectivity.