3D Cell Culture in a Self-Assembled Nanofiber Environment

The development and utilization of three-dimensional cell culture platforms has been gaining more traction. Three-dimensional culture platforms are capable of mimicking in vivo microenvironments, which provide greater physiological relevance in comparison to conventional two-dimensional cultures. Th...

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Bibliographic Details
Published in:PloS one 2016-09, Vol.11 (9), p.e0162853
Main Authors: Chai, Yi Wen, Lee, Eu Han, Gubbe, John D, Brekke, John H
Format: Article
Language:English
Subjects:
Biocompatibility
Biology
Biology and Life Sciences
Biomedical materials
Cancer
Cell adhesion
Cell adhesion & migration
Cell culture
Cell Culture Techniques
Cell Proliferation
Chitosan
Compression
Crosslinking
Cytometry
Cytotoxicity
Flow cytometry
Gene expression
HeLa Cells
Humans
Hyaluronic acid
Hydration
Hydrogels
Life sciences
Mechanical properties
Microenvironments
Microscopy, Confocal
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Mimicry
Modulus of elasticity
Nanofibers
Physical Sciences
Platforms
Polymerization
Proteins
Research and Analysis Methods
Rigidity
Self-assembly
Tissue engineering
Xenobiotics
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Summary:The development and utilization of three-dimensional cell culture platforms has been gaining more traction. Three-dimensional culture platforms are capable of mimicking in vivo microenvironments, which provide greater physiological relevance in comparison to conventional two-dimensional cultures. The majority of three-dimensional culture platforms are challenged by the lack of cell attachment, long polymerization times, and inclusion of undefined xenobiotics, and cytotoxic cross-linkers. In this study, we review the use of a highly defined material composed of naturally occurring compounds, hyaluronic acid and chitosan, known as Cell-Mate3DTM. Moreover, we provide an original measurement of Young's modulus using a uniaxial unconfined compression method to elucidate the difference in microenvironment rigidity for acellular and cellular conditions. When hydrated into a tissue-like hybrid hydrocolloid/hydrogel, Cell-Mate3DTM is a highly versatile three-dimensional culture platform that enables downstream applications such as flow cytometry, immunostaining, histological staining, and functional studies to be applied with relative ease.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0162853