A Potential Magnetic Resonance Imaging Technique Based on Chemical Exchange Saturation Transfer for In Vivo γ-Aminobutyric Acid Imaging

• Published in: PloS one 2016-10, Vol.11 (10), p.e0163765-e0163765
• Main Authors: Yan, Gen, Zhang, Tao, Dai, Zhuozhi, Yi, Meizhi, Jia, Yanlong, Nie, Tingting, Zhang, Handi, Xiao, Gang, Wu, Renhua
• Format: Article
• Language: English
• Subjects: Alzheimer's disease; Alzheimers disease; Animals; Biology and Life Sciences; Blood-brain barrier; Blood-Brain Barrier - diagnostic imaging; Blood-Brain Barrier - metabolism; Brain; Brain cancer; Brain mapping; Brain Neoplasms - diagnostic imaging; Brain Neoplasms - metabolism; Brain tumors; Choline; Creatinine; Experiments; Feasibility studies; gamma-Aminobutyric Acid - metabolism; Glutamine; Hospitals; In vivo methods and tests; Injection; Magnetic resonance; Magnetic resonance imaging; Magnetic Resonance Imaging - methods; Medical imaging; Medical research; Medicine; Medicine and Health Sciences; Metabolites; Neuroimaging; NMR; Nuclear magnetic resonance; pH effects; Phantoms, Imaging; Physical Sciences; Rats; Rats, Sprague-Dawley; Research and Analysis Methods; Resonance; Saturation; Spectrum analysis; Studies; Tumors; γ-Aminobutyric acid
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0163765

We developed a novel magnetic resonance imaging (MRI) technique based on chemical exchange saturation transfer (CEST) for GABA imaging and investigated the concentration-dependent CEST effect ofGABA in a rat model of brain tumor with blood-brain barrier (BBB) disruption. All MRI studies were performed using a 7.0-T Agilent MRI scanner. Z-spectra for GABA were acquired at 7.0 T, 37°C, and a pH of 7.0 using varying B1 amplitudes. CEST images of phantoms with different concentrations of GABA solutions (pH, 7.0) and other metabolites (glutamine, myoinositol, creatinine, and choline) were collected to investigate the concentration-dependent CEST effect of GABA and the potential contribution from other brain metabolites. CEST maps for GABA in rat brains with tumors were collected at baseline and 50 min, 1.5 h, and 2.0 h after the injection of GABA solution. The CEST effect of GABA was observed at approximately 2.75 parts per million(ppm) downfield from bulk water, and this effect increased with an increase in the B1 amplitude and remained steady after the B1 amplitude reached 6.0 μT (255 Hz). The CEST effect of GABA was proportional to the GABA concentration in vitro. CEST imaging of GABA in a rat brain with a tumor and compromised BBB showed a gradual increase in the CEST effect after GABA injection. The findings of this study demonstrate the feasibility and potential of CEST MRI with the optimal B1 amplitude, which exhibits excellent spatial and temporal resolutions, to map changes in GABA.