Performances of Four Helicobacter pylori Serological Detection Kits Using Stool Antigen Test as Gold Standard

The aim was to determine the performances of four Helicobacter pylori serological detection kits in different target groups, using Amplified IDEIA™ Hp StAR™ as gold standard. Kits studied were Rapid Immunochromatoghraphic Hexagon, Helicoblot 2.1, an EIA IgG kit and EIA IgA kit. Stool and blood sampl...

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Bibliographic Details
Published in:PloS one 2016-10, Vol.11 (10), p.e0163834-e0163834
Main Authors: Biranjia-Hurdoyal, Susheela D, Seetulsingh-Goorah, Sharmila P
Format: Article
Language:English
Subjects:
Adult
Aged
Antibiotics
Antibodies, Bacterial - blood
Antibodies, Bacterial - immunology
Antigens
Antiulcer agents
Biology and Life Sciences
Chromatography, Affinity - methods
Diabetes
Diabetes mellitus
Diabetes mellitus (non-insulin dependent)
Diabetes Mellitus, Type 2 - complications
Diabetes therapy
Enzyme-Linked Immunosorbent Assay - methods
Feces - microbiology
Female
Gastrointestinal diseases
Health sciences
Helicobacter Infections - blood
Helicobacter Infections - complications
Helicobacter Infections - diagnosis
Helicobacter Infections - immunology
Helicobacter pylori
Helicobacter pylori - immunology
Helicobacter pylori - isolation & purification
Humans
Immunoglobulin A
Immunoglobulin A - blood
Immunoglobulin A - immunology
Immunoglobulin G
Immunoglobulin G - blood
Immunoglobulin G - immunology
Immunoglobulins
Infections
Lymphoma
Male
Medicine and Health Sciences
Middle Aged
Minority & ethnic groups
People and Places
Physical Sciences
Population
Predictive Value of Tests
Reagent Kits, Diagnostic
Sampling methods
Serologic Tests - methods
Studies
Target detection
Target groups
Type 2 diabetes
Ulcers
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Summary:The aim was to determine the performances of four Helicobacter pylori serological detection kits in different target groups, using Amplified IDEIA™ Hp StAR™ as gold standard. Kits studied were Rapid Immunochromatoghraphic Hexagon, Helicoblot 2.1, an EIA IgG kit and EIA IgA kit. Stool and blood samples were collected from 162 apparently healthy participants (control) and 60 Type 2 diabetes mellitus (T2DM) patients. The performances of the four serological detection kits were found to be affected by gender, age, health status and ethnicity of the participants. In the control group, the Helicoblot 2.1 kit had the best performance (AUC = 0.85; p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0163834