Aedes aegypti D7 Saliva Protein Inhibits Dengue Virus Infection

Aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (DENV) types 1-4. Ae. aegypti transmits DENV by inoculating virus-infected saliva into host skin during probing and feeding. Ae. aegypti saliva contains over one hundred unique proteins and these pro...

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Published in:PLoS neglected tropical diseases 2016-09, Vol.10 (9), p.e0004941-e0004941
Main Authors: Conway, Michael J, Londono-Renteria, Berlin, Troupin, Andrea, Watson, Alan M, Klimstra, William B, Fikrig, Erol, Colpitts, Tonya M
Format: Article
Language:English
Subjects:
Aedes - chemistry
Aedes - virology
Animals
Aquatic insects
Biology and Life Sciences
Colleges & universities
Dengue - drug therapy
Dengue fever
Dengue virus
Dengue Virus - drug effects
Disease transmission
Female
Fractionation
Genetic aspects
Humans
Immunoglobulins
Immunology
Infections
Insect Proteins - pharmacology
Liquid chromatography
Lymph nodes
Lymphatic system
Medical research
Medicine
Medicine and Health Sciences
Mice
Mortality
Mosquitoes
Pathology
Proteins
Regulatory approval
Risk factors
Rodents
Saliva - virology
Salivary Glands - chemistry
Salivary Proteins and Peptides - pharmacology
Tropical diseases
U937 Cells
Vaccines
Vector-borne diseases
West Nile virus
Yellow fever mosquito
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description Aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (DENV) types 1-4. Ae. aegypti transmits DENV by inoculating virus-infected saliva into host skin during probing and feeding. Ae. aegypti saliva contains over one hundred unique proteins and these proteins have diverse functions, including facilitating blood feeding. Previously, we showed that Ae. aegypti salivary gland extracts (SGEs) enhanced dissemination of DENV to draining lymph nodes. In contrast, HPLC-fractionation revealed that some SGE components inhibited infection. Here, we show that D7 proteins are enriched in HPLC fractions that are inhibitory to DENV infection, and that recombinant D7 protein can inhibit DENV infection in vitro and in vivo. Further, binding assays indicate that D7 protein can directly interact with DENV virions and recombinant DENV envelope protein. These data reveal a novel role for D7 proteins, which inhibits arbovirus transmission to vertebrates through a direct interaction with virions.
doi_str_mv 10.1371/journal.pntd.0004941
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subjects Aedes - chemistry
Aedes - virology
Animals
Aquatic insects
Biology and Life Sciences
Colleges & universities
Dengue - drug therapy
Dengue fever
Dengue virus
Dengue Virus - drug effects
Disease transmission
Female
Fractionation
Genetic aspects
Humans
Immunoglobulins
Immunology
Infections
Insect Proteins - pharmacology
Liquid chromatography
Lymph nodes
Lymphatic system
Medical research
Medicine
Medicine and Health Sciences
Mice
Mortality
Mosquitoes
Pathology
Proteins
Regulatory approval
Risk factors
Rodents
Saliva - virology
Salivary Glands - chemistry
Salivary Proteins and Peptides - pharmacology
Tropical diseases
U937 Cells
Vaccines
Vector-borne diseases
West Nile virus
Yellow fever mosquito
title Aedes aegypti D7 Saliva Protein Inhibits Dengue Virus Infection
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