Association of Neuropeptide-Y (NPY) and Interleukin-1beta (IL1B), Genotype-Phenotype Correlation and Plasma Lipids with Type-II Diabetes

• Published in: PloS one 2016-10, Vol.11 (10), p.e0164437-e0164437
• Main Authors: Patel, Roma, Dwivedi, Mitesh, Mansuri, Mohmmad Shoab, Ansarullah, Laddha, Naresh C, Thakker, Ami, Ramachandran, A V, Begum, Rasheedunnisa
• Format: Article
• Language: English
• Subjects: Adult; Alleles; Beta cells; Biochemistry; Biology and Life Sciences; Blood Glucose - analysis; Body mass; Body Mass Index; Body weight; Cardiovascular disease; Case-Control Studies; Cholesterol; Correlation; Cytokines; Deoxyribonucleic acid; Diabetes; Diabetes mellitus; Diabetes Mellitus, Type 2 - genetics; Diabetes Mellitus, Type 2 - pathology; Diabetic retinopathy; DNA; Energy balance; Exons; Female; Gene Frequency; Gene polymorphism; Genetic Association Studies; Genotype; Genotyping; Haplotypes; Health risks; Humans; Insulin; Insulin resistance; Interleukin; Interleukin 1; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; Linkage analysis; Linkage Disequilibrium; Lipid metabolism; Lipids; Lipids - blood; Lipoproteins; Lipoproteins (low density); Lipoproteins, LDL - blood; Low density lipoprotein; Male; Males; Medicine and Health Sciences; Metabolism; Middle Aged; Neuropeptide Y; Neuropeptide Y - genetics; Neuropeptide Y - metabolism; Neuropeptides; Nutrition research; Patients; Phenotypes; Polymerase chain reaction; Polymorphism; Polymorphism, Single Nucleotide; Population; Promoter Regions, Genetic; Real-Time Polymerase Chain Reaction; Research and Analysis Methods; Restriction fragment length polymorphism; Risk; Risk management; RNA, Messenger - metabolism; Rodents; Satiety; Science; Transcription; Triglycerides - blood; Zoology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0164437

Neuropeptide Y (NPY) is known to play a role in the regulation of satiety, energy balance, body weight, and insulin release. Interleukin-1beta (IL1B) has been associated with loss of beta-cell mass in type-II diabetes (TIID). The present study attempts to investigate the association of NPY exon2 +1128 T/C (Leu7Pro; rs16139), NPY promoter -399 T/C (rs16147) and IL1B -511 C/T (rs16944) polymorphisms with TIID and their correlation with plasma lipid levels, BMI, and IL1B transcript levels. PCR-RFLP was used for genotyping these polymorphisms in a case-control study involving 558 TIID patients and 1085 healthy age-matched controls from Gujarat. Linkage disequilibrium and haplotype analysis of the NPY polymorphic sites were performed to assess their association with TIID. IL1B transcript levels in PBMCs were also assessed in 108 controls and 101 patients using real-time PCR. Our results show significant association of both structural and promoter polymorphisms of NPY (p