In Vivo Evaluation of the Visual Pathway in Streptozotocin-Induced Diabetes by Diffusion Tensor MRI and Contrast Enhanced MRI

• Published in: PloS one 2016-10, Vol.11 (10), p.e0165169-e0165169
• Main Authors: Kancherla, Swarupa, Kohler, William J, van der Merwe, Yolandi, Chan, Kevin C
• Format: Article
• Language: English
• Subjects: Acids; Animal experimentation; Animal models; Animals; Anisotropy; Anterograde transport; Bioengineering; Biology and Life Sciences; Blindness; Blood sugar; Brain; Brain research; Chromium; Citric acid; Colliculus; Contrast agents; Contrast Media; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - diagnostic imaging; Diabetic retinopathy; Diffusion; Diffusion Magnetic Resonance Imaging - methods; Diffusivity; Drug dosages; Engineering schools; Humans; Hyperglycemia; Injection; Integrity; Laboratories; Lateral geniculate nucleus; Magnetic resonance; Magnetic resonance imaging; Manganese; Medical imaging; Medicine; Medicine and Health Sciences; Neuroimaging; Neurosciences; NMR; Nuclear magnetic resonance; Optic nerve; Organic acids; Rats; Rats, Sprague-Dawley; Research and Analysis Methods; Retina; Retinopathy; Social Sciences; Streptozocin; Studies; Substantia alba; Superior colliculus; Transport; Type 2 diabetes; Visual impairment; Visual observation; Visual pathways; Visual Pathways - pathology; Visual perception; Visual system
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0165169

Visual function has been shown to deteriorate prior to the onset of retinopathy in some diabetic patients and experimental animal models. This suggests the involvement of the brain's visual system in the early stages of diabetes. In this study, we tested this hypothesis by examining the integrity of the visual pathway in a diabetic rat model using in vivo multi-modal magnetic resonance imaging (MRI). Ten-week-old Sprague-Dawley rats were divided into an experimental diabetic group by intraperitoneal injection of 65 mg/kg streptozotocin in 0.01 M citric acid, and a sham control group by intraperitoneal injection of citric acid only. One month later, diffusion tensor MRI (DTI) was performed to examine the white matter integrity in the brain, followed by chromium-enhanced MRI of retinal integrity and manganese-enhanced MRI of anterograde manganese transport along the visual pathway. Prior to MRI experiments, the streptozotocin-induced diabetic rats showed significantly smaller weight gain and higher blood glucose level than the control rats. DTI revealed significantly lower fractional anisotropy and higher radial diffusivity in the prechiasmatic optic nerve of the diabetic rats compared to the control rats. No apparent difference was observed in the axial diffusivity of the optic nerve, the chromium enhancement in the retina, or the manganese enhancement in the lateral geniculate nucleus and superior colliculus between groups. Our results suggest that streptozotocin-induced diabetes leads to early injury in the optic nerve when no substantial change in retinal integrity or anterograde transport along the visual pathways was observed in MRI using contrast agent enhancement. DTI may be a useful tool for detecting and monitoring early pathophysiological changes in the visual system of experimental diabetes non-invasively.