Bumetanide, an Inhibitor of NKCC1 (Na-K-2Cl Cotransporter Isoform 1), Enhances Propofol-Induced Loss of Righting Reflex but Not Its Immobilizing Actions in Neonatal Rats

• Published in: PloS one 2016-10, Vol.11 (10), p.e0164125-e0164125
• Main Authors: Koyama, Yukihide, Andoh, Tomio, Kamiya, Yoshinori, Miyazaki, Tomoyuki, Maruyama, Koichi, Kariya, Takayuki, Goto, Takahisa
• Format: Article
• Language: English
• Subjects: Adenosine monophosphate; Anesthesia; Anesthesiology; Animals; Animals, Newborn; Behavior, Animal - drug effects; Biology and Life Sciences; Bumetanide; Bumetanide - pharmacology; Central nervous system; Cerebral Cortex - drug effects; Cerebral Cortex - metabolism; Cerebral Cortex - pathology; Chloride transport; Comparative analysis; CREB-Binding Protein - metabolism; Critical care; Cyclic AMP; Developmental stages; Drug dosages; GABA; Hippocampus; Hippocampus - drug effects; Hippocampus - metabolism; Hippocampus - pathology; Hypotheses; Immobilization; Inhibitors; Medicine; Medicine and Health Sciences; Midazolam; Neonates; Newborn babies; Phenols (Class of compounds); Posture; Propofol; Propofol - pharmacology; Protein binding; Rats; Rats, Sprague-Dawley; Receiving; Reflex, Righting - drug effects; Research and Analysis Methods; Righting reflex; Rodents; Signaling; Solute Carrier Family 12, Member 2 - chemistry; Solute Carrier Family 12, Member 2 - metabolism; Spinal cord; Thalamus; University graduates
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0164125

Gamma-aminobutyric acid (GABA) has been shown to induce excitation on immature neurons due to increased expression of Na+-K+-2Cl- co-transporter isoform 1 (NKCC1), and the transition of GABAergic signaling from excitatory to inhibitory occurs before birth in the rat spinal cord and spreads rostrally according to the developmental changes in cation-chloride co-transporter expression. We previously showed that midazolam activates the hippocampal CA3 area and induces less sedation in neonatal rats compared with adolescent rats in an NKCC1-dependent manner. In the present study, we tested the hypothesis that propofol-induced loss of righting reflex (LORR) but not immobilizing actions are modulated by NKCC1-dependent mechanisms and reduced in neonatal rats compared with adolescent rats. We estimated neuronal activity in the cortex, hippocampus and thalamus after propofol administration with or without bumetanide, an NKCC1 inhibitor, by immunostaining of phosphorylated cyclic adenosine monophosphate-response element binding protein (pCREB). We studied effects of bumetanide on propofol-induced LORR and immobilizing actions in postnatal day 7 and 28 (P7 and P28) rats. The pCREB expression in the cortex (P = 0.001) and hippocampus (P = 0.01) was significantly greater in the rats receiving propofol only than in the rats receiving propofol plus bumetanide at P 7. Propofol-induced LORR or immobilizing effects did not differ significantly between P7 and P28. Bumetanide significantly enhanced propofol-induced LORR (P = 0.031) but not immobilization in P7 rats. These results are partially consistent with our hypothesis. They suggest that propofol may activate the rostral but not caudal central nervous system dependently on NKCC1, and these differential actions may underlie the different properties of sedative and immobilizing actions observed in neonatal rats.