Changes to the Aqueous Humor Proteome during Glaucoma

To investigate the aqueous humor proteome in patients with glaucoma and a control group. Aqueous humor was obtained from five human donors diagnosed with primary open angle glaucoma (POAG) and five age- and sex-matched controls undergoing cataract surgery. Quantitative proteome analysis of the aqueo...

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Bibliographic Details
Published in:PloS one 2016-10, Vol.11 (10), p.e0165314
Main Authors: Kaeslin, Martha Andrea, Killer, Hanspeter Ezriel, Fuhrer, Cyril Adrian, Zeleny, Nauke, Huber, Andreas Robert, Neutzner, Albert
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Antioxidants
Antioxidants (Nutrients)
Aqueous Humor - metabolism
Biology and Life Sciences
Case-Control Studies
Cataracts
Cell adhesion & migration
Cholesterol
Cloning
Comparative analysis
Consortia
Eye surgery
Female
Glaucoma
Glaucoma - metabolism
Health aspects
Humans
Inflammation
Inflammatory response
Laboratories
Male
Mass spectrometry
Mass spectroscopy
Medical research
Medicine and Health Sciences
Metabolism
Middle Aged
Neurophysiology
Ontology
Ophthalmology
Optic nerve
Oxidative stress
Patients
Proteins
Proteolysis
Proteome
Proteomes
Scientific imaging
Surgery
Up-Regulation
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Summary:To investigate the aqueous humor proteome in patients with glaucoma and a control group. Aqueous humor was obtained from five human donors diagnosed with primary open angle glaucoma (POAG) and five age- and sex-matched controls undergoing cataract surgery. Quantitative proteome analysis of the aqueous humor by hyper reaction monitoring mass spectrometry (HRM-MS) based on SWATH technology was performed. Expression levels of 87 proteins were found to be different between glaucomatous and control aqueous humor. Of the 87 proteins, 34 were significantly upregulated, whereas 53 proteins were downregulated in the aqueous humor from glaucoma patients compared to controls. Differentially expressed proteins were found to be involved in cholesterol-related, inflammatory, metabolic, antioxidant as well as proteolysis-related processes. Glaucoma leads to profound changes to the aqueous humor proteome consistent with an altered metabolic state, an inflammatory response and impaired antioxidant defense.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0165314