Microbial Community Evolution Is Significantly Impacted by the Use of Calcium Isosaccharinic Acid as an Analogue for the Products of Alkaline Cellulose Degradation

• Published in: PloS one 2016-11, Vol.11 (11), p.e0165832-e0165832
• Main Authors: Kyeremeh, Isaac A, Charles, Christopher J, Rout, Simon P, Laws, Andrew P, Humphreys, Paul N
• Format: Article
• Language: English
• Subjects: Acetogenesis; Acids; Acinetobacter; Bacteria - classification; Bacteria - genetics; Bacteria - pathogenicity; Biology; Biology and Life Sciences; Calcium; Calcium - chemistry; Calcium - metabolism; Carbon sources; Cellulose; Clostridiales; Clostridium; Community composition; Computer and Information Sciences; Consortia; Degradation; DNA, Bacterial - analysis; Gram-positive bacteria; Hazardous waste management industry; High-Throughput Nucleotide Sequencing; Intrusion; Leachates; Medicine and Health Sciences; Metabolism; Methanogenesis; Microbial Consortia; Microbiology; Microbiomes; Microorganisms; Migration; Next-generation sequencing; Nitrogen; Nuclear engineering; Nuclear safety; Physical Sciences; Physiological aspects; Radioactive pollution; Radioactive Waste; Radioactive wastes; Radioisotopes; Reactors; Research and Analysis Methods; Sequence Analysis, DNA; Social Sciences; Soil Microbiology; Stereoisomerism; Studies; Substrates; Sugar Acids - chemistry; Sugar Acids - metabolism; Taxa; United Kingdom; Waste disposal; Waste Management - methods
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0165832

Diasteriomeric isosaccharinic acid (ISA) is an important consideration within safety assessments for the disposal of the United Kingdoms' nuclear waste legacy, where it may potentially influence radionuclide migration. Since the intrusion of micro-organisms may occur within a disposal concept, the impact of ISA may be impacted by microbial metabolism. Within the present study we have established two polymicrobial consortia derived from a hyperalkaline soil. Here, α-ISA and a diatereomeric mix of ISAs' were used as a sole carbon source, reflecting two common substrates appearing within the literature. The metabolism of ISA within these two consortia was similar, where ISA degradation resulted in the acetogenesis and hydrogenotrophic methanogenesis. The chemical data obtained confirm that the diastereomeric nature of ISA is likely to have no impact on its metabolism within alkaline environments. High throughput sequencing of the original soil showed a diverse community which, in the presence of ISA allowed for the dominance the Clostridiales associated taxa with Clostridium clariflavum prevalent. Further taxonomic investigation at the genus level showed that there was in fact a significant difference (p = 0.004) between the two community profiles. Our study demonstrates that the selection of carbon substrate is likely to have a significant impact on microbial community composition estimations, which may have implications with respect to a safety assessment of an ILW-GDF.