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Investigation of Factors Affecting Aerobic and Respiratory Growth in the Oxygen-Tolerant Strain Lactobacillus casei N87
Aerobic and respiratory cultivations provide benefits for some lactic acid bacteria (LAB). Growth, metabolites, enzymatic activities (lactate dehydrogenase; pyruvate and NADH oxidases, NADH peroxidase; catalase), antioxidant capability and stress tolerance of Lactobacillus casei N87 were evaluated i...
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| Published in: | PloS one 2016-11, Vol.11 (11), p.e0164065-e0164065 |
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| creator | Ianniello, Rocco G Zotta, Teresa Matera, Attilio Genovese, Francesco Parente, Eugenio Ricciardi, Annamaria |
| description | Aerobic and respiratory cultivations provide benefits for some lactic acid bacteria (LAB). Growth, metabolites, enzymatic activities (lactate dehydrogenase; pyruvate and NADH oxidases, NADH peroxidase; catalase), antioxidant capability and stress tolerance of Lactobacillus casei N87 were evaluated in anaerobic, aerobic and respiratory (aerobiosis with heme and menaquinone supplementation) batch cultivations with different dissolved oxygen (DO) concentrations. The expression of pox (pyruvate oxidase) and cydABCD operon (cytochrome bd oxidase complex) was quantified by quantitative Real Time polymerase chain reaction. Respiration increased biomass production compared to anaerobiosis and unsupplemented aerobiosis, and altered the central metabolism rerouting pyruvate away from lactate accumulation. All enzymatic activities, except lactate dehydrogenase, were higher in respiratory cultures, while unsupplemented aerobiosis with 60% of DO promoted H2O2 and free radical accumulation. Respiration improved the survival to oxidative and freeze-drying stresses, while significant numbers of dead, damaged and viable but not cultivable cells were found in unsupplemented aerobic cultures (60% DO). Analysis of gene expression suggested that the activation of aerobic and respiratory pathways occurred during the exponential growth phase, and that O2 and hemin induced, respectively, the transcription of pox and cydABCD genes. Respiratory cultivation might be a natural strategy to improve functional and technological properties of L. casei. |
| doi_str_mv | 10.1371/journal.pone.0164065 |
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Growth, metabolites, enzymatic activities (lactate dehydrogenase; pyruvate and NADH oxidases, NADH peroxidase; catalase), antioxidant capability and stress tolerance of Lactobacillus casei N87 were evaluated in anaerobic, aerobic and respiratory (aerobiosis with heme and menaquinone supplementation) batch cultivations with different dissolved oxygen (DO) concentrations. The expression of pox (pyruvate oxidase) and cydABCD operon (cytochrome bd oxidase complex) was quantified by quantitative Real Time polymerase chain reaction. Respiration increased biomass production compared to anaerobiosis and unsupplemented aerobiosis, and altered the central metabolism rerouting pyruvate away from lactate accumulation. All enzymatic activities, except lactate dehydrogenase, were higher in respiratory cultures, while unsupplemented aerobiosis with 60% of DO promoted H2O2 and free radical accumulation. Respiration improved the survival to oxidative and freeze-drying stresses, while significant numbers of dead, damaged and viable but not cultivable cells were found in unsupplemented aerobic cultures (60% DO). Analysis of gene expression suggested that the activation of aerobic and respiratory pathways occurred during the exponential growth phase, and that O2 and hemin induced, respectively, the transcription of pox and cydABCD genes. Respiratory cultivation might be a natural strategy to improve functional and technological properties of L. casei.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0164065</identifier><identifier>PMID: 27812097</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accumulation ; Acids ; Aerobiosis - drug effects ; Air flow ; Anaerobiosis ; Anaerobiosis - drug effects ; Antioxidants ; Antioxidants - metabolism ; Bacteria ; Biological Transport - drug effects ; Biology and Life Sciences ; Catalase ; Comparative analysis ; Cultivation ; Cytochrome ; Cytochrome bd ; Dehydrogenase ; Dehydrogenases ; Dissolved oxygen ; Earth Sciences ; Enzymatic activity ; Freeze drying ; Gene expression ; Gene Expression Regulation, Bacterial - drug effects ; Genes ; Growth ; Heme ; Heme - pharmacology ; Hemin ; Hydrogen peroxide ; Investigations ; Kinetics ; L-Lactate dehydrogenase ; Laboratories ; Lactate dehydrogenase ; Lactic acid ; Lactic acid bacteria ; Lactobacilli ; Lactobacillus casei ; Lactobacillus casei - drug effects ; Lactobacillus casei - genetics ; Lactobacillus casei - growth & development ; Lactobacillus casei - metabolism ; Lactobacillus plantarum ; Lactococcus lactis ; Medicine and Health Sciences ; Menaquinones ; Metabolism ; Metabolites ; NADH ; NADH peroxidase ; Nicotinamide adenine dinucleotide ; Operon - genetics ; Oxidase ; Oxidases ; Oxidative stress ; Oxidative Stress - drug effects ; Oxygen ; Oxygen - metabolism ; Peroxidase ; Physical Sciences ; Polymerase chain reaction ; Prokaryotes ; Pyruvate oxidase ; Pyruvic acid ; Respiration ; Statistics ; Stress response ; Supplements ; Transcription ; Transcription (Genetics) ; Vitamin K 2 - pharmacology</subject><ispartof>PloS one, 2016-11, Vol.11 (11), p.e0164065-e0164065</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><rights>2016 Ianniello et al. 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Growth, metabolites, enzymatic activities (lactate dehydrogenase; pyruvate and NADH oxidases, NADH peroxidase; catalase), antioxidant capability and stress tolerance of Lactobacillus casei N87 were evaluated in anaerobic, aerobic and respiratory (aerobiosis with heme and menaquinone supplementation) batch cultivations with different dissolved oxygen (DO) concentrations. The expression of pox (pyruvate oxidase) and cydABCD operon (cytochrome bd oxidase complex) was quantified by quantitative Real Time polymerase chain reaction. Respiration increased biomass production compared to anaerobiosis and unsupplemented aerobiosis, and altered the central metabolism rerouting pyruvate away from lactate accumulation. All enzymatic activities, except lactate dehydrogenase, were higher in respiratory cultures, while unsupplemented aerobiosis with 60% of DO promoted H2O2 and free radical accumulation. 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Respiratory cultivation might be a natural strategy to improve functional and technological properties of L. casei.</description><subject>Accumulation</subject><subject>Acids</subject><subject>Aerobiosis - drug effects</subject><subject>Air flow</subject><subject>Anaerobiosis</subject><subject>Anaerobiosis - drug effects</subject><subject>Antioxidants</subject><subject>Antioxidants - metabolism</subject><subject>Bacteria</subject><subject>Biological Transport - drug effects</subject><subject>Biology and Life Sciences</subject><subject>Catalase</subject><subject>Comparative analysis</subject><subject>Cultivation</subject><subject>Cytochrome</subject><subject>Cytochrome bd</subject><subject>Dehydrogenase</subject><subject>Dehydrogenases</subject><subject>Dissolved oxygen</subject><subject>Earth Sciences</subject><subject>Enzymatic activity</subject><subject>Freeze drying</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial - drug effects</subject><subject>Genes</subject><subject>Growth</subject><subject>Heme</subject><subject>Heme - pharmacology</subject><subject>Hemin</subject><subject>Hydrogen peroxide</subject><subject>Investigations</subject><subject>Kinetics</subject><subject>L-Lactate dehydrogenase</subject><subject>Laboratories</subject><subject>Lactate dehydrogenase</subject><subject>Lactic acid</subject><subject>Lactic acid bacteria</subject><subject>Lactobacilli</subject><subject>Lactobacillus casei</subject><subject>Lactobacillus casei - drug effects</subject><subject>Lactobacillus casei - genetics</subject><subject>Lactobacillus casei - growth & development</subject><subject>Lactobacillus casei - metabolism</subject><subject>Lactobacillus plantarum</subject><subject>Lactococcus lactis</subject><subject>Medicine and Health Sciences</subject><subject>Menaquinones</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>NADH</subject><subject>NADH peroxidase</subject><subject>Nicotinamide adenine dinucleotide</subject><subject>Operon - genetics</subject><subject>Oxidase</subject><subject>Oxidases</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ianniello, Rocco G</au><au>Zotta, Teresa</au><au>Matera, Attilio</au><au>Genovese, Francesco</au><au>Parente, Eugenio</au><au>Ricciardi, Annamaria</au><au>Virolle, Marie-Joelle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of Factors Affecting Aerobic and Respiratory Growth in the Oxygen-Tolerant Strain Lactobacillus casei N87</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-11-03</date><risdate>2016</risdate><volume>11</volume><issue>11</issue><spage>e0164065</spage><epage>e0164065</epage><pages>e0164065-e0164065</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Aerobic and respiratory cultivations provide benefits for some lactic acid bacteria (LAB). Growth, metabolites, enzymatic activities (lactate dehydrogenase; pyruvate and NADH oxidases, NADH peroxidase; catalase), antioxidant capability and stress tolerance of Lactobacillus casei N87 were evaluated in anaerobic, aerobic and respiratory (aerobiosis with heme and menaquinone supplementation) batch cultivations with different dissolved oxygen (DO) concentrations. The expression of pox (pyruvate oxidase) and cydABCD operon (cytochrome bd oxidase complex) was quantified by quantitative Real Time polymerase chain reaction. Respiration increased biomass production compared to anaerobiosis and unsupplemented aerobiosis, and altered the central metabolism rerouting pyruvate away from lactate accumulation. All enzymatic activities, except lactate dehydrogenase, were higher in respiratory cultures, while unsupplemented aerobiosis with 60% of DO promoted H2O2 and free radical accumulation. Respiration improved the survival to oxidative and freeze-drying stresses, while significant numbers of dead, damaged and viable but not cultivable cells were found in unsupplemented aerobic cultures (60% DO). Analysis of gene expression suggested that the activation of aerobic and respiratory pathways occurred during the exponential growth phase, and that O2 and hemin induced, respectively, the transcription of pox and cydABCD genes. Respiratory cultivation might be a natural strategy to improve functional and technological properties of L. casei.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27812097</pmid><doi>10.1371/journal.pone.0164065</doi><tpages>e0164065</tpages><orcidid>https://orcid.org/0000-0001-8228-6804</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-11, Vol.11 (11), p.e0164065-e0164065 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1835969836 |
| source | Publicly Available Content Database; PubMed Central |
| subjects | Accumulation Acids Aerobiosis - drug effects Air flow Anaerobiosis Anaerobiosis - drug effects Antioxidants Antioxidants - metabolism Bacteria Biological Transport - drug effects Biology and Life Sciences Catalase Comparative analysis Cultivation Cytochrome Cytochrome bd Dehydrogenase Dehydrogenases Dissolved oxygen Earth Sciences Enzymatic activity Freeze drying Gene expression Gene Expression Regulation, Bacterial - drug effects Genes Growth Heme Heme - pharmacology Hemin Hydrogen peroxide Investigations Kinetics L-Lactate dehydrogenase Laboratories Lactate dehydrogenase Lactic acid Lactic acid bacteria Lactobacilli Lactobacillus casei Lactobacillus casei - drug effects Lactobacillus casei - genetics Lactobacillus casei - growth & development Lactobacillus casei - metabolism Lactobacillus plantarum Lactococcus lactis Medicine and Health Sciences Menaquinones Metabolism Metabolites NADH NADH peroxidase Nicotinamide adenine dinucleotide Operon - genetics Oxidase Oxidases Oxidative stress Oxidative Stress - drug effects Oxygen Oxygen - metabolism Peroxidase Physical Sciences Polymerase chain reaction Prokaryotes Pyruvate oxidase Pyruvic acid Respiration Statistics Stress response Supplements Transcription Transcription (Genetics) Vitamin K 2 - pharmacology |
| title | Investigation of Factors Affecting Aerobic and Respiratory Growth in the Oxygen-Tolerant Strain Lactobacillus casei N87 |
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