Microplasma Induced Cell Morphological Changes and Apoptosis of Ex Vivo Cultured Human Anterior Lens Epithelial Cells - Relevance to Capsular Opacification

Inducing selective or targeted cell apoptosis without affecting large number of neighbouring cells remains a challenge. A plausible method for treatment of posterior capsular opacification (PCO) due to remaining lens epithelial cells (LECs) by reactive chemistry induced by localized single electrode...

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Published in:PloS one 2016-11, Vol.11 (11), p.e0165883-e0165883
Main Authors: Recek, Nina, Andjelić, Sofija, Hojnik, Nataša, Filipič, Gregor, Lazović, Saša, Vesel, Alenka, Primc, Gregor, Mozetič, Miran, Hawlina, Marko, Petrovski, Goran, Cvelbar, Uroš
Format: Article
Language:English
Subjects:
Apoptosis
Atmospheric pressure
Biology
Biology and Life Sciences
Capsule Opacification - therapy
Caspase
Caspase-8
Cataracts
Cells, Cultured
Cellular structure
Charged particles
CXCR4 protein
Deoxyribonucleic acid
Discharge
DNA
DNA fragmentation
Electrodes
Engineering
Epithelial cells
Epithelial Cells - cytology
Equipment Design
Eye surgery
Free radicals
Gas flow
Hospitals
Humans
Lens Capsule, Crystalline - cytology
Lenses
Lipid peroxidation
Lipids
Medicine and Health Sciences
Micromanipulation - instrumentation
Microscopy
Morphology
Ophthalmology
Physical Sciences
Physics
Plasma
Plasma Gases - administration & dosage
Plasma Gases - therapeutic use
Stem cells
Surgery
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cited_by cdi_FETCH-LOGICAL-c725t-4c506c3461ce26beb9218f67ceccbb5440768c17a69246743cc9bf24b690e2e23
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container_title PloS one
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creator Recek, Nina
Andjelić, Sofija
Hojnik, Nataša
Filipič, Gregor
Lazović, Saša
Vesel, Alenka
Primc, Gregor
Mozetič, Miran
Hawlina, Marko
Petrovski, Goran
Cvelbar, Uroš
description Inducing selective or targeted cell apoptosis without affecting large number of neighbouring cells remains a challenge. A plausible method for treatment of posterior capsular opacification (PCO) due to remaining lens epithelial cells (LECs) by reactive chemistry induced by localized single electrode microplasma discharge at top of a needle-like glass electrode with spot size ~3 μm is hereby presented. The focused and highly-localized atmospheric pressure microplasma jet with electrode discharge could induce a dose-dependent apoptosis in selected and targeted individual LECs, which could be confirmed by real-time monitoring of the morphological and structural changes at cellular level. Direct cell treatment with microplasma inside the medium appeared more effective in inducing apoptosis (caspase 8 positivity and DNA fragmentation) at a highly targeted cell level compared to treatment on top of the medium (indirect treatment). Our results show that single cell specific micropipette plasma can be used to selectively induce demise in LECs which remain in the capsular bag after cataract surgery and thus prevent their migration (CXCR4 positivity) to the posterior lens capsule and PCO formation.
doi_str_mv 10.1371/journal.pone.0165883
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identifier ISSN: 1932-6203
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1932-6203
language eng
recordid cdi_plos_journals_1838211894
source Publicly Available Content Database; PubMed Central
subjects Apoptosis
Atmospheric pressure
Biology
Biology and Life Sciences
Capsule Opacification - therapy
Caspase
Caspase-8
Cataracts
Cells, Cultured
Cellular structure
Charged particles
CXCR4 protein
Deoxyribonucleic acid
Discharge
DNA
DNA fragmentation
Electrodes
Engineering
Epithelial cells
Epithelial Cells - cytology
Equipment Design
Eye surgery
Free radicals
Gas flow
Hospitals
Humans
Lens Capsule, Crystalline - cytology
Lenses
Lipid peroxidation
Lipids
Medicine and Health Sciences
Micromanipulation - instrumentation
Microscopy
Morphology
Ophthalmology
Physical Sciences
Physics
Plasma
Plasma Gases - administration & dosage
Plasma Gases - therapeutic use
Stem cells
Surgery
title Microplasma Induced Cell Morphological Changes and Apoptosis of Ex Vivo Cultured Human Anterior Lens Epithelial Cells - Relevance to Capsular Opacification
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