Primary Postnatal Dorsal Root Ganglion Culture from Conventionally Slaughtered Calves

Neurological disorders in ruminants have an important impact on veterinary health, but very few host-specific in vitro models have been established to study diseases affecting the nervous system. Here we describe a primary neuronal dorsal root ganglia (DRG) culture derived from calves after being co...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2016-12, Vol.11 (12), p.e0168228-e0168228
Main Authors: Fadda, A, Bärtschi, M, Hemphill, A, Widmer, H R, Zurbriggen, A, Perona, P, Vidondo, B, Oevermann, A
Format: Article
Language:English
Subjects:
Animal diseases
Animals
Axonogenesis
Biology and Life Sciences
Calves
Cattle
Cell culture
Cell survival
Cells, Cultured
Coculture Techniques
Dietary supplements
Dorsal root ganglia
Food consumption
Ganglia
Ganglia, Spinal - pathology
Growth factors
Immunofluorescence
Innovations
Kinases
Medicine and Health Sciences
Microscopy, Electron, Transmission
Nervous system
Nervous system diseases
Neurological diseases
Neurons
Pain
Physical Sciences
Rodents
Ruminantia
Spinal cord
Studies
Veterinary medicine
Viability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Neurological disorders in ruminants have an important impact on veterinary health, but very few host-specific in vitro models have been established to study diseases affecting the nervous system. Here we describe a primary neuronal dorsal root ganglia (DRG) culture derived from calves after being conventionally slaughtered for food consumption. The study focuses on the in vitro characterization of bovine DRG cell populations by immunofluorescence analysis. The effects of various growth factors on neuron viability, neurite outgrowth and arborisation were evaluated by morphological analysis. Bovine DRG neurons are able to survive for more than 4 weeks in culture. GF supplementation is not required for neuronal survival and neurite outgrowth. However, exogenously added growth factors promote neurite outgrowth. DRG cultures from regularly slaughtered calves represent a promising and sustainable host specific model for the investigation of pain and neurological diseases in bovines.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0168228