Circulating Microvesicles Are Elevated Acutely following Major Burns Injury and Associated with Clinical Severity

Microvesicles are cell-derived signaling particles emerging as important mediators and biomarkers of systemic inflammation, but their production in severe burn injury patients has not been described. In this pilot investigation, we measured circulating microvesicle levels following severe burns, wit...

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Bibliographic Details
Published in:PloS one 2016-12, Vol.11 (12), p.e0167801-e0167801
Main Authors: O'Dea, Kieran P, Porter, John R, Tirlapur, Nikhil, Katbeh, Umar, Singh, Suveer, Handy, Jonathan M, Takata, Masao
Format: Article
Language:English
Subjects:
Adult
Age
Aged
Aged, 80 and over
Aging
Analysis
Bioindicators
Biology and Life Sciences
Biomarkers
Burns
Burns - complications
Burns - pathology
Cancer
Cell-Derived Microparticles - pathology
Consent
Cytometry
Endothelial cells
Endothelial Cells - pathology
Female
Flow cytometry
Granulocytes - pathology
Health aspects
Hospitals
Humans
Inflammation
Inflammatory response
Injuries
Intensive care
Leukocytes
Leukocytes (granulocytic)
Leukocytes - pathology
Male
Medicine
Medicine and Health Sciences
Middle Aged
Monocytes
Monocytes - pathology
Mortality
Neutrophils
Pain
Pathological physiology
Patients
Research and Analysis Methods
Risk factors
ROC Curve
Sepsis
Sepsis - complications
Sepsis - pathology
Signaling
Subpopulations
Surgery
Systemic inflammatory response syndrome
Systemic Inflammatory Response Syndrome - complications
Systemic Inflammatory Response Syndrome - pathology
Thermal injury
Trauma
Young Adult
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Summary:Microvesicles are cell-derived signaling particles emerging as important mediators and biomarkers of systemic inflammation, but their production in severe burn injury patients has not been described. In this pilot investigation, we measured circulating microvesicle levels following severe burns, with severe sepsis patients as a comparator group. We hypothesized that levels of circulating vascular cell-derived microvesicles are elevated acutely following burns injury, mirroring clinical severity due to the early onset and prevalence of systemic inflammatory response syndrome (SIRS) in these patients. Blood samples were obtained from patients with moderate to severe thermal injury burns, with severe sepsis, and from healthy volunteers. Circulating microvesicles derived from total leukocytes, granulocytes, monocytes, and endothelial cells were quantified in plasma by flow cytometry. All circulating microvesicle subpopulations were elevated in burns patients on day of admission (day 0) compared to healthy volunteers (leukocyte-microvesicles: 3.5-fold, p = 0.005; granulocyte-microvesicles: 12.8-fold, p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0167801