Comprehensive Virus Detection Using Next Generation Sequencing in Grapevine Vascular Tissues of Plants Obtained from the Wine Regions of Bohemia and Moravia (Czech Republic)

Comprehensive next generation sequencing virus detection was used to detect the whole spectrum of viruses and viroids in selected grapevines from the Czech Republic. The novel NGS approach was based on sequencing libraries of small RNA isolated from grapevine vascular tissues. Eight previously parti...

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Published in:PloS one 2016-12, Vol.11 (12), p.e0167966-e0167966
Main Authors: Eichmeier, Aleš, Komínková, Marcela, Komínek, Petr, Baránek, Miroslav
Format: Article
Language:English
Subjects:
Analysis
Biology and Life Sciences
Czech Republic
DNA sequencing
DNA, Viral - chemistry
Farms
Gene sequencing
Genetics
Grapevines
High-Throughput Nucleotide Sequencing - methods
Horticulture
Infections
Microorganisms
Nucleotide sequencing
Pathogens
Plant tissues
Plant viruses
Plant Viruses - isolation & purification
Research and Analysis Methods
Ribonucleic acid
RNA
Sequence Analysis, DNA - methods
Vascular system of plants
Vineyards
Viroids
Viroids - isolation & purification
Viruses
Vitaceae
Vitis - virology
Vitis vinifera
Wine
Wineries & vineyards
Wines
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description Comprehensive next generation sequencing virus detection was used to detect the whole spectrum of viruses and viroids in selected grapevines from the Czech Republic. The novel NGS approach was based on sequencing libraries of small RNA isolated from grapevine vascular tissues. Eight previously partially-characterized grapevines of diverse varieties were selected and subjected to analysis: Chardonnay, Laurot, Guzal Kara, and rootstock Kober 125AA from the Moravia wine-producing region; plus Müller-Thurgau and Pinot Noir from the Bohemia wine-producing region, both in the Czech Republic. Using next generation sequencing of small RNA, the presence of 8 viruses and 2 viroids were detected in a set of eight grapevines; therefore, confirming the high effectiveness of the technique in plant virology and producing results supporting previous data on multiple infected grapevines in Czech vineyards. Among the pathogens detected, the Grapevine rupestris vein feathering virus and Grapevine yellow speckle viroid 1 were recorded in the Czech Republic for the first time.
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The novel NGS approach was based on sequencing libraries of small RNA isolated from grapevine vascular tissues. Eight previously partially-characterized grapevines of diverse varieties were selected and subjected to analysis: Chardonnay, Laurot, Guzal Kara, and rootstock Kober 125AA from the Moravia wine-producing region; plus Müller-Thurgau and Pinot Noir from the Bohemia wine-producing region, both in the Czech Republic. Using next generation sequencing of small RNA, the presence of 8 viruses and 2 viroids were detected in a set of eight grapevines; therefore, confirming the high effectiveness of the technique in plant virology and producing results supporting previous data on multiple infected grapevines in Czech vineyards. Among the pathogens detected, the Grapevine rupestris vein feathering virus and Grapevine yellow speckle viroid 1 were recorded in the Czech Republic for the first time.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27959951</pmid><doi>10.1371/journal.pone.0167966</doi><tpages>e0167966</tpages><oa>free_for_read</oa></addata></record>
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subjects Analysis
Biology and Life Sciences
Czech Republic
DNA sequencing
DNA, Viral - chemistry
Farms
Gene sequencing
Genetics
Grapevines
High-Throughput Nucleotide Sequencing - methods
Horticulture
Infections
Microorganisms
Nucleotide sequencing
Pathogens
Plant tissues
Plant viruses
Plant Viruses - isolation & purification
Research and Analysis Methods
Ribonucleic acid
RNA
Sequence Analysis, DNA - methods
Vascular system of plants
Vineyards
Viroids
Viroids - isolation & purification
Viruses
Vitaceae
Vitis - virology
Vitis vinifera
Wine
Wineries & vineyards
Wines
title Comprehensive Virus Detection Using Next Generation Sequencing in Grapevine Vascular Tissues of Plants Obtained from the Wine Regions of Bohemia and Moravia (Czech Republic)
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