Ocular Blood Flow Measurements in Healthy White Subjects Using Laser Speckle Flowgraphy

To assess the feasibility and reliability of Laser Speckle Flowgraphy (LSFG) to measure ocular perfusion in a sample of healthy white subjects and to elucidate the age-dependence of the parameters obtained. This cross-sectional study included 80 eyes of 80 healthy, non-smoking white subjects of West...

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Published in:PloS one 2016-12, Vol.11 (12), p.e0168190-e0168190
Main Authors: Luft, Nikolaus, Wozniak, Piotr A, Aschinger, Gerold C, Fondi, Klemens, Bata, Ahmed M, Werkmeister, René M, Schmidl, Doreen, Witkowska, Katarzyna J, Bolz, Matthias, Garhöfer, Gerhard, Schmetterer, Leopold
Format: Article
Language:English
Subjects:
Adolescent
Adult
Age
Age Factors
Aged
Analysis
Bioindicators
Biology and Life Sciences
Biomarkers
Biomedical engineering
Blood
Blood flow
Blood Flow Velocity - physiology
Blood Pressure - physiology
Correlation coefficient
Correlation coefficients
Cross-Sectional Studies
Diabetic retinopathy
Dilation
Engineering
Engineering and Technology
European Continental Ancestry Group
Eye (anatomy)
Feasibility studies
Female
Flow velocity
Glaucoma
Health physics
Healthy Volunteers
Hemodynamics
Hospitals
Humans
Hypotheses
Image analysis
Image processing
Image Processing, Computer-Assisted
Image quality
Laser speckle
Laser-Doppler Flowmetry
Lasers
Male
Measurement
Medicine and Health Sciences
Microcirculation - physiology
Middle Aged
Optic Disk - blood supply
Optic nerve
Optics
Patients
People and Places
Perfusion
Pharmacology
Physical Sciences
Physics
Pigmentation
Pupil - physiology
Regional Blood Flow - physiology
Reliability analysis
Reproducibility
Reproducibility of Results
Sex Factors
Smoking
Time Factors
Velocity
Young Adult
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cited_by cdi_FETCH-LOGICAL-c791t-c7e58fdda6060c8e674343ec591034b52a16b13ee76780d20d630164eccb96263
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container_end_page e0168190
container_issue 12
container_start_page e0168190
container_title PloS one
container_volume 11
creator Luft, Nikolaus
Wozniak, Piotr A
Aschinger, Gerold C
Fondi, Klemens
Bata, Ahmed M
Werkmeister, René M
Schmidl, Doreen
Witkowska, Katarzyna J
Bolz, Matthias
Garhöfer, Gerhard
Schmetterer, Leopold
description To assess the feasibility and reliability of Laser Speckle Flowgraphy (LSFG) to measure ocular perfusion in a sample of healthy white subjects and to elucidate the age-dependence of the parameters obtained. This cross-sectional study included 80 eyes of 80 healthy, non-smoking white subjects of Western European descent between 19 and 79 years of age. A commercial LSFG instrument was applied to measure ocular blood flow at the optic nerve head (ONH) three successive times before and after pharmacological pupil dilation. The mean blur rate (MBR), a measure of relative blood flow velocity, was obtained for different regions of the ONH. Eight parameters of ocular perfusion derived from the pulse-waveform analysis of MBR including blowout time (BOT) and falling rate (FR) were also recorded. Artifact-free LSFG images meeting the quality criteria for automated image analysis were obtainable in 93.8% without pupil dilation and in 98.8% with pharmacological pupil dilation. Measurements of MBR showed excellent repeatability with intraclass correlation coefficients ≥ 0.937 and were barely affected by pupil dilation. The majority of pulse-waveform derived variables exhibited equally high repeatability. MBR-related blood flow indices exhibited significant age dependence (p
doi_str_mv 10.1371/journal.pone.0168190
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blood supply</topic><topic>Optic nerve</topic><topic>Optics</topic><topic>Patients</topic><topic>People and Places</topic><topic>Perfusion</topic><topic>Pharmacology</topic><topic>Physical Sciences</topic><topic>Physics</topic><topic>Pigmentation</topic><topic>Pupil - physiology</topic><topic>Regional Blood Flow - physiology</topic><topic>Reliability analysis</topic><topic>Reproducibility</topic><topic>Reproducibility of Results</topic><topic>Sex Factors</topic><topic>Smoking</topic><topic>Time Factors</topic><topic>Velocity</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luft, Nikolaus</creatorcontrib><creatorcontrib>Wozniak, Piotr A</creatorcontrib><creatorcontrib>Aschinger, Gerold C</creatorcontrib><creatorcontrib>Fondi, Klemens</creatorcontrib><creatorcontrib>Bata, Ahmed M</creatorcontrib><creatorcontrib>Werkmeister, René M</creatorcontrib><creatorcontrib>Schmidl, Doreen</creatorcontrib><creatorcontrib>Witkowska, Katarzyna J</creatorcontrib><creatorcontrib>Bolz, Matthias</creatorcontrib><creatorcontrib>Garhöfer, Gerhard</creatorcontrib><creatorcontrib>Schmetterer, Leopold</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale in Context : Opposing Viewpoints</collection><collection>Gale in Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luft, Nikolaus</au><au>Wozniak, Piotr A</au><au>Aschinger, Gerold C</au><au>Fondi, Klemens</au><au>Bata, Ahmed M</au><au>Werkmeister, René M</au><au>Schmidl, Doreen</au><au>Witkowska, Katarzyna J</au><au>Bolz, Matthias</au><au>Garhöfer, Gerhard</au><au>Schmetterer, Leopold</au><au>Chan-Ling, Tailoi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ocular Blood Flow Measurements in Healthy White Subjects Using Laser Speckle Flowgraphy</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-12-13</date><risdate>2016</risdate><volume>11</volume><issue>12</issue><spage>e0168190</spage><epage>e0168190</epage><pages>e0168190-e0168190</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>To assess the feasibility and reliability of Laser Speckle Flowgraphy (LSFG) to measure ocular perfusion in a sample of healthy white subjects and to elucidate the age-dependence of the parameters obtained. This cross-sectional study included 80 eyes of 80 healthy, non-smoking white subjects of Western European descent between 19 and 79 years of age. A commercial LSFG instrument was applied to measure ocular blood flow at the optic nerve head (ONH) three successive times before and after pharmacological pupil dilation. The mean blur rate (MBR), a measure of relative blood flow velocity, was obtained for different regions of the ONH. Eight parameters of ocular perfusion derived from the pulse-waveform analysis of MBR including blowout time (BOT) and falling rate (FR) were also recorded. Artifact-free LSFG images meeting the quality criteria for automated image analysis were obtainable in 93.8% without pupil dilation and in 98.8% with pharmacological pupil dilation. Measurements of MBR showed excellent repeatability with intraclass correlation coefficients ≥ 0.937 and were barely affected by pupil dilation. The majority of pulse-waveform derived variables exhibited equally high repeatability. MBR-related blood flow indices exhibited significant age dependence (p&lt;0.001). FR (r = 0.747, p&lt;0.001) and BOT (r = -0.714, p&lt;0.001) most strongly correlated with age. LSFG represents a reliable method for the quantitative assessment of ocular blood flow in white subjects. Our data affirms that the LSFG-derived variables FR and BOT may be useful biomarkers for age-related changes in ocular perfusion.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27959905</pmid><doi>10.1371/journal.pone.0168190</doi><tpages>e0168190</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1932-6203
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issn 1932-6203
1932-6203
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subjects Adolescent
Adult
Age
Age Factors
Aged
Analysis
Bioindicators
Biology and Life Sciences
Biomarkers
Biomedical engineering
Blood
Blood flow
Blood Flow Velocity - physiology
Blood Pressure - physiology
Correlation coefficient
Correlation coefficients
Cross-Sectional Studies
Diabetic retinopathy
Dilation
Engineering
Engineering and Technology
European Continental Ancestry Group
Eye (anatomy)
Feasibility studies
Female
Flow velocity
Glaucoma
Health physics
Healthy Volunteers
Hemodynamics
Hospitals
Humans
Hypotheses
Image analysis
Image processing
Image Processing, Computer-Assisted
Image quality
Laser speckle
Laser-Doppler Flowmetry
Lasers
Male
Measurement
Medicine and Health Sciences
Microcirculation - physiology
Middle Aged
Optic Disk - blood supply
Optic nerve
Optics
Patients
People and Places
Perfusion
Pharmacology
Physical Sciences
Physics
Pigmentation
Pupil - physiology
Regional Blood Flow - physiology
Reliability analysis
Reproducibility
Reproducibility of Results
Sex Factors
Smoking
Time Factors
Velocity
Young Adult
title Ocular Blood Flow Measurements in Healthy White Subjects Using Laser Speckle Flowgraphy
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