The Study on Biological Function of Keratin 26, a Novel Member of Liaoning Cashmere Goat Keratin Gene Family

• Published in: PloS one 2016-12, Vol.11 (12), p.e0168015-e0168015
• Main Authors: Jin, Mei, Wang, Jing, Chu, Ming-Xing, Piao, Jun, Piao, Jing-Ai, Zhao, Feng-Qin
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Biology and Life Sciences; Bone morphogenetic proteins; Cashmere; Cell cycle; Cellular signal transduction; Change detection; Fibroblast growth factor 5; Fibroblasts; Follicles; Gene expression; Gene Expression Regulation - physiology; Genetic aspects; Goats; Growth; Growth factors; Hair; Hair Follicle - metabolism; Hybridization; Immunofluorescence; Immunohistochemistry; Insulin-like growth factor I; Insulin-like growth factors; Keratin; Keratins - biosynthesis; Kinases; Life sciences; Ligands; Medicine and Health Sciences; Mutation; Noggin protein; Properties; Proteins; Real time; Researchers; RNA-mediated interference; Signal transduction; Signaling; Skin; Wool - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0168015

In our research, we explored the relationship between Keratin 26 and the regulation of fine hair, BMP signaling pathway, MT, FGF5, and IGF-I. The result of hybridization in situ revealed that Keratin 26 was specially expressed in cortex of skin hair follicles; the result of immunohistochemistry indicated that Keratin 26 was expressed in internal root sheath, external root sheath. Then, Real-time quantitative PCR results showed that relative expressive quantity of Keratin 26 was 1.08 or 3.3 × greater in secondary follicle than primary follicle during anagen or catagen; the difference during anagen was not remarkable (p>0.05), however, that of catagen was extremely significant (p