Thin-Film Transistor-Based Biosensors for Determining Stoichiometry of Biochemical Reactions

The enzyme kinetic in a biochemical reaction is critical to scientific research and drug discovery but can hardly be determined experimentally from enzyme assays. In this work, a charge-current transducer (a transistor) is proposed to evaluate the status of biochemical reaction by monitoring the ele...

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Bibliographic Details
Published in:PloS one 2016-12, Vol.11 (12), p.e0169094-e0169094
Main Authors: Wang, Yi-Wen, Chen, Ting-Yang, Yang, Tsung-Han, Chang, Cheng-Chung, Yang, Tsung-Lin, Lo, Yu-Hwa, Huang, Jian-Jang
Format: Article
Language:English
Subjects:
Applied physics
Biochemistry
Biology and Life Sciences
Biosensing Techniques - instrumentation
Biosensors
Chemical reactions
Computer engineering
Electric Conductivity
Electric properties
Engineering and Technology
Enzyme kinetics
Enzymes
Gold
Kinetics
Malate
Metabolism
NAD
NAD - metabolism
NADH
Nicotinamide adenine dinucleotide
Oxaloacetic Acid - metabolism
Photonics
Physical Sciences
Research and Analysis Methods
Science
Scientific imaging
Scientific research
Semiconductor devices
Semiconductors
Sensors
Stoichiometry
Thin film transistors
Thin films
Transistors
Transistors, Electronic
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Summary:The enzyme kinetic in a biochemical reaction is critical to scientific research and drug discovery but can hardly be determined experimentally from enzyme assays. In this work, a charge-current transducer (a transistor) is proposed to evaluate the status of biochemical reaction by monitoring the electrical charge changes. Using the malate-aspartate shuttle as an example, a thin-film transistor (TFT)-based biosensor with an extended gold pad is demonstrated to detect the biochemical reaction between NADH and NAD+. The drain current change indicates the status of chemical equilibrium and stoichiometry.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0169094