Cry3Bb1-Resistant Western Corn Rootworm, Diabrotica virgifera virgifera (LeConte) Does Not Exhibit Cross-Resistance to DvSnf7 dsRNA

There is a continuing need to express new insect control compounds in transgenic maize against western corn rootworm, Diabrotica virgifera virgifera (LeConte) (WCR). In this study three experiments were conducted to determine cross-resistance between the new insecticidal DvSnf7 dsRNA, and Bacillus t...

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Bibliographic Details
Published in:PloS one 2017-01, Vol.12 (1), p.e0169175-e0169175
Main Authors: Moar, William, Khajuria, Chitvan, Pleau, Michael, Ilagan, Oliver, Chen, Mao, Jiang, Changjian, Price, Paula, McNulty, Brian, Clark, Thomas, Head, Graham
Format: Article
Language:English
Subjects:
Animals
Bacillus thuringiensis
Bacillus thuringiensis - genetics
Bacillus thuringiensis - metabolism
Bactrocera dorsalis
Beetles
Bioassays
Biological Assay
Biology and Life Sciences
Coleoptera
Coleoptera - drug effects
Coleoptera - genetics
Coleoptera - pathogenicity
Colonies
Corn
Cross-resistance
Diabrotica
Diabrotica undecimpunctata howardi
Diabrotica virgifera
Diabrotica virgifera virgifera
Double-stranded RNA
Durability
Emergence
Endotoxins - pharmacology
Exhibitions
Gene expression
Genes
Genetic aspects
Genetically modified crops
Insect control
Insecticide resistance
Insecticide Resistance - genetics
Insecticide Resistance - physiology
Insects
Leptinotarsa decemlineata
Medicine and Health Sciences
Newborn babies
Physiological aspects
Plants (botany)
Plants, Genetically Modified - parasitology
Population studies
Populations
Proteins
Research and Analysis Methods
RNA, Double-Stranded - genetics
RNA, Double-Stranded - physiology
Transgenic
Trends
Zea mays
Zea mays - parasitology
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Summary:There is a continuing need to express new insect control compounds in transgenic maize against western corn rootworm, Diabrotica virgifera virgifera (LeConte) (WCR). In this study three experiments were conducted to determine cross-resistance between the new insecticidal DvSnf7 dsRNA, and Bacillus thuringiensis (Bt) Cry3Bb1; used to control WCR since 2003, with field-evolved resistance being reported. Laboratory susceptible and Cry3Bb1-resistant WCR were evaluated against DvSnf7 dsRNA in larval diet-incorporation bioassays. Additionally, the susceptibility of seven field and one field-derived WCR populations to DvSnf7 (and Cry3Bb1) was assessed in larval diet-overlay bioassays. Finally, beetle emergence of laboratory susceptible and Cry3Bb1-resistant WCR was evaluated with maize plants in the greenhouse expressing Cry3Bb1, Cry34Ab1/Cry35Ab1, or DvSnf7 dsRNA singly, or in combination. The Cry3Bb1-resistant colony had slight but significantly (2.7-fold; P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0169175