Physiological Characterization and Comparative Transcriptome Analysis of White and Green Leaves of Ananas comosus var. bracteatus

• Published in: PloS one 2017-01, Vol.12 (1), p.e0169838-e0169838
• Main Authors: Li, Xia, Kanakala, Surapathrudu, He, Yehua, Zhong, Xiaolan, Yu, Sanmiao, Li, Ruixue, Sun, Lingxia, Ma, Jun
• Format: Article
• Language: English
• Subjects: Albinism; Analysis; Ananas - genetics; Ananas - metabolism; Ananas comosus; Annotations; Biology and Life Sciences; Biosynthesis; Carotenoids; Chlorophyll; Chlorophyll - metabolism; Chloroplasts; Coloration; Enzymatic activity; Enzyme activity; Fruits; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genetic aspects; Genomes; High-Throughput Nucleotide Sequencing; Hydroxymethylbilane synthase; Landscape architecture; Leaf coloration; Leaves; Leukocytes; Metabolism; Metabolites; Molecular modelling; Photosynthesis; Physical Sciences; Physiological aspects; Physiology; Pineapples; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Proteins - genetics; Plants (botany); Research and analysis methods; Ribonucleic acid; RNA; Studies; Transcription; Transcription (Genetics); Transcriptome - genetics
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0169838

Leaf coloration is one of the most important and attractive characteristics of Ananas comosus var. bracteatus. The chimeric character is not stable during the in vitro tissue culturing. Many regenerated plants lost economic values for the loss of the chimeric character of leaves. In order to reveal the molecular mechanisms involved in the albino phenotype of the leaf cells, the physiological and transcriptional differences between complete white (CWh) and green (CGr) leaf cells of A. comosus var. bracteatus were analyzed. A total of 1,431 differentially expressed unigenes (DEGs) in CGr and CWh leaves were identified using RNA-seq. A comparison to the COG, GO and KEGG annotations revealed DEGs involved in chlorophyll biosynthesis, chloroplast development and photosynthesis. Furthermore, the measurement of main precursors of chlorophyll in the CWh leaves confirmed that the rate-limiting step in chlorophyll biosynthesis, and thus the cause of the albino phenotype of the white cells, was the conversion of pyrrole porphobilinogen (PBG) to uroporphyrinogen III (Uro III). The enzyme activity of porphobilinogen deaminase (PBGD) and uroporporphyrinogn III synthase (UROS), which catalyze the transition of PBG to Uro III, was significantly decreased in the CWh leaves. Our data showed the transcriptional differences between the CWh and CGr plants and characterized key steps in chlorophyll biosynthesis of the CWh leaves. These results contribute to our understanding of the mechanisms and regulation of pigment biosynthesis in the CWh leaf cells of A. comosus var. bracteatus.