The Spleen Is an Ideal Site for Inducing Transplanted Islet Graft Expansion in Mice

Alternative islet transplantation sites have the potential to reduce the marginal number of islets required to ameliorate hyperglycemia in recipients with diabetes. Previously, we reported that T cell leukemia homeobox 1 (Tlx1)+ stem cells in the spleen effectively regenerated into insulin-producing...

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Bibliographic Details
Published in:PloS one 2017-01, Vol.12 (1), p.e0170899
Main Authors: Itoh, Takeshi, Nishinakamura, Hitomi, Kumano, Kenjiro, Takahashi, Hiroyuki, Kodama, Shohta
Format: Article
Language:English
Subjects:
Animals
Biology and Life Sciences
Chemokines - blood
Cloning
Diabetes
Diabetes mellitus
Diabetes Mellitus, Experimental - therapy
Gene Expression Regulation
Glucose
Glucose Tolerance Test
Grafting
Grafts
Homeobox
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Hospitals
Hyperglycemia
Inflammation
Inflammation - pathology
Insulin
Islet cell transplantation
Islet cells
Islets of Langerhans Transplantation
Kidney - physiology
Kidneys
Leukemia
Liver - physiology
Liver transplants
Lymphocytes T
Male
Medicine
Medicine and Health Sciences
Mice
Mice, Inbred C57BL
Pancreas
Pancreatic islet transplantation
Physiological aspects
Portal vein
Portal Vein - physiology
Rodents
Spleen
Spleen - physiology
Stem cell transplantation
Stem cells
Streptozocin
Studies
Syngeneic grafts
Time Factors
Tlx1 protein
Transplantation
Transplants & implants
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Summary:Alternative islet transplantation sites have the potential to reduce the marginal number of islets required to ameliorate hyperglycemia in recipients with diabetes. Previously, we reported that T cell leukemia homeobox 1 (Tlx1)+ stem cells in the spleen effectively regenerated into insulin-producing cells in the pancreas of non-obese diabetic mice with end-stage disease. Thus, we investigated the spleen as a potential alternative islet transplantation site. Streptozotocin-induced diabetic C57BL/6 mice received syngeneic islets into the portal vein (PV), beneath the kidney capsule (KC), or into the spleen (SP). The marginal number of islets by PV, KC, or SP was 200, 100, and 50, respectively. Some plasma inflammatory cytokine levels in the SP group were significantly lower than those of the PV group after receiving a marginal number of islets, indicating reduced inflammation in the SP group. Insulin contents were increased 280 days after islet transplantation compared with those immediately following transplantation (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0170899