Identification of a vacuolar proton channel that triggers the bioluminescent flash in dinoflagellates

• Published in: PloS one 2017-02, Vol.12 (2), p.e0171594
• Main Authors: Rodriguez, Juan D, Haq, Saddef, Bachvaroff, Tsvetan, Nowak, Kristine F, Nowak, Scott J, Morgan, Deri, Cherny, Vladimir V, Sapp, Maredith M, Bernstein, Steven, Bolt, Andrew, DeCoursey, Thomas E, Place, Allen R, Smith, Susan M E
• Format: Article
• Language: English
• Subjects: Action potential; Biology and Life Sciences; Bioluminescence; Biophysics; Cell cycle; Cell Membrane - metabolism; Cellular biology; Channel gating; Compartments; Confocal; Confocal microscopy; Depolarization; Dinoflagellates; Dinoflagellida - metabolism; Electric potential; Environmental science; Fluorescence; Hydrogen-Ion Concentration; Ion Channel Gating; Ion channels; Ion Channels - metabolism; Light; Light emission; Lingulodinium polyedrum; Mass Spectrometry; Medicine and Health Sciences; Microorganisms; Microscopy; Peptides; pH effects; Physical Sciences; Physiological aspects; Physiology; Proteomics; Protons; Pyrrophycophyta; Research and Analysis Methods; Signal transduction; Vacuoles - metabolism; Voltage; Western blotting; Zinc; Zinc - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0171594

In 1972, J. Woodland Hastings and colleagues predicted the existence of a proton selective channel (HV1) that opens in response to depolarizing voltage across the vacuole membrane of bioluminescent dinoflagellates and conducts protons into specialized luminescence compartments (scintillons), thereby causing a pH drop that triggers light emission. HV1 channels were subsequently identified and demonstrated to have important functions in a multitude of eukaryotic cells. Here we report a predicted protein from Lingulodinium polyedrum that displays hallmark properties of bona fide HV1, including time-dependent opening with depolarization, perfect proton selectivity, and characteristic ΔpH dependent gating. Western blotting and fluorescence confocal microscopy of isolated L. polyedrum scintillons immunostained with antibody to LpHV1 confirm LpHV1's predicted organellar location. Proteomics analysis demonstrates that isolated scintillon preparations contain peptides that map to LpHV1. Finally, Zn2+ inhibits both LpHV1 proton current and the acid-induced flash in isolated scintillons. These results implicate LpHV1 as the voltage gated proton channel that triggers bioluminescence in L. polyedrum, confirming Hastings' hypothesis. The same channel likely mediates the action potential that communicates the signal along the tonoplast to the scintillon.