Purification, characterization, and antitumor activity of a novel glucan from the fruiting bodies of Coriolus Versicolor

• Published in: PloS one 2017-02, Vol.12 (2), p.e0171270-e0171270
• Main Authors: Awadasseid, Annoor, Hou, Jie, Gamallat, Yaser, Xueqi, Shang, Eugene, Kuugbee D, Musa Hago, Ahmed, Bamba, Djibril, Meyiah, Abdo, Gift, Chiwala, Xin, Yi
• Format: Article
• Language: English
• Subjects: Animals; Anticancer properties; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Antitumor activity; Apoptosis; Basidiomycota - chemistry; Biochemistry; Biological Products - isolation & purification; Biological Products - pharmacology; Biology and Life Sciences; Biotechnology; Body weight; Cancer; Cancer therapies; Carbohydrates; Care and treatment; Cell cycle; Cell Line, Tumor; Chromatography; Coriolus; Coriolus versicolor; Disease Models, Animal; Ethanol; Female; Fruit bodies; Fruiting Bodies, Fungal - chemistry; Glucan; Glucans; Glucans - chemistry; Glucans - isolation & purification; Glucans - pharmacology; Health aspects; Hot water; Humans; Immune system; Immunomodulation; In vivo methods and tests; Inflammatory bowel disease; Leukemia; Lymphocytes - drug effects; Lymphocytes - immunology; Lymphocytes - metabolism; Macrophages - drug effects; Macrophages - immunology; Macrophages - metabolism; Magnetic Resonance Spectroscopy; Medicine and Health Sciences; Mice; Molecular weight; Mushrooms; Physical Sciences; Plant extracts; Polymers; Proteins; Purification; Research and analysis methods; Sarcoma; Spectroscopy, Fourier Transform Infrared; Spectrum analysis; Spleen; Structural analysis; Thymus; Water purification; Xenograft Model Antitumor Assays
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0171270

Cancer is one of the most common causes of deaths worldwide. Herein, we report an efficient natural anticancer glucan (CVG) extracted from Coriolus Versicolar (CV). CVG was extracted by the hot water extraction method followed by ethanol precipitation and purified using gas exclusion chromatography. Structural analysis revealed that CVG has a linear α-glucan chain composed of only (1→ 6)-α-D-Glcp. The antitumor activity of CVG on Sarcoma-180 cells was investigated in vitro and in vivo. Mice were treated with three doses of CVG (40, 100, 200 mg/kg body weight) for 9 days. Tumor weight, relative spleen, thymus weight, and lymphocyte proliferation were studied. A significant increase (P< 0.01) in relative spleen and thymus weight and a decrease (P< 0.01) in tumor weight at the doses of 100 and 200 mg/kg were observed. The results obtained demonstrate CVG has antitumor activity towards Sarcoma-180 cells by its immunomodulation activity.