The Drosophila speciation factor HMR localizes to genomic insulator sites

Hybrid incompatibility between Drosophila melanogaster and D. simulans is caused by a lethal interaction of the proteins encoded by the Hmr and Lhr genes. In D. melanogaster the loss of HMR results in mitotic defects, an increase in transcription of transposable elements and a deregulation of hetero...

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Bibliographic Details
Published in:PloS one 2017-02, Vol.12 (2), p.e0171798-e0171798
Main Authors: Gerland, Thomas Andreas, Sun, Bo, Smialowski, Pawel, Lukacs, Andrea, Thomae, Andreas Walter, Imhof, Axel
Format: Article
Language:English
Subjects:
Animals
Binding sites
Biodiversity
Biology and Life Sciences
Cell culture
Cell cycle
Chromatin
Chromosomes
Deoxyribonucleic acid
Deregulation
DNA
Drosophila
Drosophila - genetics
Drosophila melanogaster
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Drosophila Proteins - physiology
Drosophila simulans
Evolution & development
Gene expression
Gene mutation
Genes
Genetic aspects
Genetic Speciation
Genetics
Genome, Insect
Genomes
Hybridization, Genetic
Immunoprecipitation
Incompatibility
Insects
Insulators
Larvae
Localization
Molecular biology
Molecular modelling
Ovaries
Physical Sciences
Proteins
Research and Analysis Methods
Speciation
Tissue culture
Transcription
Transcription (Genetics)
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Summary:Hybrid incompatibility between Drosophila melanogaster and D. simulans is caused by a lethal interaction of the proteins encoded by the Hmr and Lhr genes. In D. melanogaster the loss of HMR results in mitotic defects, an increase in transcription of transposable elements and a deregulation of heterochromatic genes. To better understand the molecular mechanisms that mediate HMR's function, we measured genome-wide localization of HMR in D. melanogaster tissue culture cells by chromatin immunoprecipitation. Interestingly, we find HMR localizing to genomic insulator sites that can be classified into two groups. One group belongs to gypsy insulators and another one borders HP1a bound regions at active genes. The transcription of the latter group genes is strongly affected in larvae and ovaries of Hmr mutant flies. Our data suggest a novel link between HMR and insulator proteins, a finding that implicates a potential role for genome organization in the formation of species.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0171798