Characterisation and genome sequence of the lytic Acinetobacter baumannii bacteriophage vB_AbaS_Loki

Acinetobacter baumannii has emerged as an important nosocomial pathogen in healthcare and community settings. While over 100 of Acinetobacter phages have been described in the literature, relatively few have been sequenced. This work describes the characterisation and genome annotation of a new lyti...

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Bibliographic Details
Published in:PloS one 2017-02, Vol.12 (2), p.e0172303-e0172303
Main Authors: Turner, Dann, Wand, Matthew E, Briers, Yves, Lavigne, Rob, Sutton, J Mark, Reynolds, Darren M
Format: Article
Language:English
Subjects:
Acinetobacter
Acinetobacter baumannii
Acinetobacter baumannii - isolation & purification
Acinetobacter baumannii - virology
Acinetobacter Infections - microbiology
Activated sludge
Analysis
Annotations
Antibiotics
Bacteria
Bacteriophages - genetics
Bacteriophages - isolation & purification
Bacteriophages - pathogenicity
Bioinformatics
Biology and Life Sciences
Burkholderia
Calcium
DNA sequencing
DNA, Viral - genetics
Drug resistance
Gene sequencing
Genes
Genome, Viral
Genomes
Health care
Hospitals
Host range
Humans
Infections
Laboratories
Medicine and Health Sciences
Molecular biology
Morphology
Nonsense mutation
Nosocomial infection
Nucleotide sequence
Open reading frames
Paracoccus
Phages
Phylogeny
Physical Sciences
Proteins
Pseudomonas
Public health
Sequence Analysis, DNA
Sewage sludge
Stop codon
Viral Plaque Assay
Viral Proteins - genetics
Virus Attachment
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Summary:Acinetobacter baumannii has emerged as an important nosocomial pathogen in healthcare and community settings. While over 100 of Acinetobacter phages have been described in the literature, relatively few have been sequenced. This work describes the characterisation and genome annotation of a new lytic Acinetobacter siphovirus, vB_AbaS_Loki, isolated from activated sewage sludge. Sequencing revealed that Loki encapsulates a 41,308 bp genome, encoding 51 predicted open reading frames. Loki is most closely related to Acinetobacter phage IME_AB3 and more distantly related to Burkholderia phage KL1, Paracoccus phage vB_PmaS_IMEP1 and Pseudomonas phages vB_Pae_Kakheti25, vB_PaeS_SCH_Ab26 and PA73. Loki is characterised by a narrow host range, among the 40 Acinetobacter isolates tested, productive infection was only observed for the propagating host, A. baumannii ATCC 17978. Plaque formation was found to be dependent upon the presence of Ca2+ ions and adsorption to host cells was abolished upon incubation with a mutant of ATCC 17978 encoding a premature stop codon in lpxA. The complete genome sequence of vB_AbaS_Loki was deposited in the European Nucleotide Archive (ENA) under the accession number LN890663.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0172303