Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis

• Published in: PloS one 2017-02, Vol.12 (2), p.e0171796-e0171796
• Main Authors: Komegae, Evilin Naname, Souza, Tais Aparecida Matozo, Grund, Lidiane Zito, Lima, Carla, Lopes-Ferreira, Monica
• Format: Article
• Language: English
• Subjects: Adaptive immunity; Amino Acid Sequence; Analysis; Animals; Antigens; Antigens, Differentiation - genetics; Antigens, Differentiation - immunology; Biology and Life Sciences; Brazil; Care and treatment; Cell adhesion & migration; Central nervous system; Cuprizone; Demyelination; Development and progression; Disease Models, Animal; Encephalomyelitis, Autoimmune, Experimental - chemically induced; Encephalomyelitis, Autoimmune, Experimental - drug therapy; Encephalomyelitis, Autoimmune, Experimental - immunology; Encephalomyelitis, Autoimmune, Experimental - pathology; Experimental allergic encephalomyelitis; Female; Fish; Fish Venoms - chemistry; Gelatinase B; Gene Expression Regulation; Health aspects; Helper cells; Immunity; Immunologic Factors - isolation & purification; Immunologic Factors - pharmacology; Infiltration; Inflammation; Interferon-gamma - genetics; Interferon-gamma - immunology; Interleukin 10; Interleukin-10 - genetics; Interleukin-10 - immunology; Interleukin-17 - genetics; Interleukin-17 - immunology; Laboratories; Lymphocytes; Lymphocytes T; Macrophages; Macrophages - drug effects; Macrophages - immunology; Macrophages - pathology; Maintenance; Matrix Metalloproteinase 9 - genetics; Matrix Metalloproteinase 9 - immunology; Medicine and Health Sciences; Mice; Multiple sclerosis; Multiple Sclerosis - drug therapy; Multiple Sclerosis - immunology; Multiple Sclerosis - pathology; Myelination; Nervous system; NMR; Nuclear magnetic resonance; Peptides; Peptides - isolation & purification; Peptides - pharmacology; Perciformes - metabolism; Prevalence studies (Epidemiology); Spinal cord; Spinal Cord - drug effects; Spinal Cord - immunology; Spinal Cord - pathology; T-Lymphocytes, Regulatory - drug effects; T-Lymphocytes, Regulatory - immunology; T-Lymphocytes, Regulatory - pathology; Th1 Cells - drug effects; Th1 Cells - immunology; Th1 Cells - pathology; Th17 Cells - drug effects; Th17 Cells - immunology; Th17 Cells - pathology; Venom; γ-Interferon
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0171796

The pathological condition of multiple sclerosis (MS) relies on innate and adaptive immunity. New types of agents that beneficially modify the course of MS, stopping the progression and repairing the damage appear promising. Here, we studied TnP, a small stable synthetic peptide derived from fish venom in the control of inflammation and demyelination in experimental autoimmune encephalomyelitis as prophylactic treatment. TnP decreased the number of the perivascular infiltrates in spinal cord, and the activity of MMP-9 by F4/80+ macrophages were decreased after different regimen treatments. TnP reduces in the central nervous system the infiltration of IFN-γ-producing Th1 and IL-17A-producing Th17 cells. Also, treatment with therapeutic TnP promotes the emergence of functional Treg in the central nervous system entirely dependent on IL-10. Therapeutic TnP treatment accelerates the remyelination process in a cuprizone model of demyelination. These findings support the beneficial effects of TnP and provides a new therapeutic opportunity for the treatment of MS.