Analysis of Spo0M function in Bacillus subtilis

Spo0M has been previously reported as a regulator of sporulation in Bacillus subtilis; however, little is known about the mechanisms through which it participates in sporulation, and there is no information to date that relates this protein to other processes in the bacterium. In this work we presen...

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Published in:PloS one 2017-02, Vol.12 (2), p.e0172737-e0172737
Main Authors: Vega-Cabrera, Luz Adriana, Guerrero, Adán, Rodríguez-Mejía, José Luis, Tabche, María Luisa, Wood, Christopher D, Gutiérrez-Rios, Rosa-María, Merino, Enrique, Pardo-López, Liliana
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacillus subtilis - genetics
Bacillus subtilis - growth & development
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biofilms
Bioinformatics
Biology and Life Sciences
Cell division
Cell Division - genetics
Chromosomes
Cytoskeletal Proteins - biosynthesis
Cytoskeletal Proteins - genetics
Cytoskeleton
Genetic aspects
Homology
Life cycle engineering
Life Cycle Stages - genetics
Life cycles
Localization
Medicine and Health Sciences
Microscopy
Mutation
Phase transitions
Phosphatase
Phosphorylation
Protein interaction
Protein Interaction Maps
Protein-protein interactions
Proteins
Proteomics
Research and Analysis Methods
RNA polymerase
Spores, Bacterial - genetics
Spores, Bacterial - growth & development
Sporulation
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cited_by cdi_FETCH-LOGICAL-c725t-6e0fd436bd2cb2b8682cbe38aced1b02067ea0adb0874db85ff322af23966dc73
cites cdi_FETCH-LOGICAL-c725t-6e0fd436bd2cb2b8682cbe38aced1b02067ea0adb0874db85ff322af23966dc73
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container_title PloS one
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creator Vega-Cabrera, Luz Adriana
Guerrero, Adán
Rodríguez-Mejía, José Luis
Tabche, María Luisa
Wood, Christopher D
Gutiérrez-Rios, Rosa-María
Merino, Enrique
Pardo-López, Liliana
description Spo0M has been previously reported as a regulator of sporulation in Bacillus subtilis; however, little is known about the mechanisms through which it participates in sporulation, and there is no information to date that relates this protein to other processes in the bacterium. In this work we present evidence from proteomic, protein-protein interaction, morphological, subcellular localization microscopy and bioinformatics studies which indicate that Spo0M function is not necessarily restricted to sporulation, and point towards its involvement in other stages of the vegetative life cycle. In the current study, we provide evidence that Spo0M interacts with cytoskeletal proteins involved in cell division, which suggest a function additional to that previously described in sporulation. Spo0M expression is not restricted to the transition phase or sporulation; rather, its expression begins during the early stages of growth and Spo0M localization in B. subtilis depends on the bacterial life cycle and could be related to an additional proposed function. This is supported by our discovery of homologs in a broad distribution of bacterial genera, even in non-sporulating species. Our work paves the way for re-evaluation of the role of Spo0M in bacterial cell.
doi_str_mv 10.1371/journal.pone.0172737
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subjects Bacillus subtilis
Bacillus subtilis - genetics
Bacillus subtilis - growth & development
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biofilms
Bioinformatics
Biology and Life Sciences
Cell division
Cell Division - genetics
Chromosomes
Cytoskeletal Proteins - biosynthesis
Cytoskeletal Proteins - genetics
Cytoskeleton
Genetic aspects
Homology
Life cycle engineering
Life Cycle Stages - genetics
Life cycles
Localization
Medicine and Health Sciences
Microscopy
Mutation
Phase transitions
Phosphatase
Phosphorylation
Protein interaction
Protein Interaction Maps
Protein-protein interactions
Proteins
Proteomics
Research and Analysis Methods
RNA polymerase
Spores, Bacterial - genetics
Spores, Bacterial - growth & development
Sporulation
title Analysis of Spo0M function in Bacillus subtilis
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