Novel form of miR-29b suppresses bleomycin-induced pulmonary fibrosis

MicroRNA 29b (miR-29b) replacement therapy is effective for suppressing fibrosis in a mouse model. However, to develop clinical applications for miRNA mimics, the side effects of nucleic acid drugs have to be addressed. In this study, we focused on miRNA mimics in order to develop therapies for idio...

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Bibliographic Details
Published in:PloS one 2017-02, Vol.12 (2), p.e0171957-e0171957
Main Authors: Yamada, Yuko, Takanashi, Masakatsu, Sudo, Katsuko, Ueda, Shinobu, Ohno, Shin-Ichiro, Kuroda, Masahiko
Format: Article
Language:English
Subjects:
Administration, Inhalation
Analysis
Animals
Biology and Life Sciences
Bleomycin
Bleomycin - adverse effects
Cancer therapies
Collagen
Dosage and administration
Drug therapy
Drugs
Fibroblasts - metabolism
Fibrosis
Gene expression
Growth factors
Health aspects
Hydroxyproline - analysis
Lung diseases
Lungs
Male
Mice
Mice, Inbred C57BL
MicroRNA
MicroRNAs
MicroRNAs - therapeutic use
miRNA
NIH 3T3 Cells
Nucleic acids
Pathology
Pulmonary fibrosis
Pulmonary Fibrosis - chemically induced
Pulmonary Fibrosis - drug therapy
Research and Analysis Methods
Respiratory system agents
Ribonucleic acid
RNA
Side effects
Signal Transduction
Systematic review
Therapeutic applications
Toll-Like Receptors - metabolism
Transforming Growth Factor beta - pharmacology
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Summary:MicroRNA 29b (miR-29b) replacement therapy is effective for suppressing fibrosis in a mouse model. However, to develop clinical applications for miRNA mimics, the side effects of nucleic acid drugs have to be addressed. In this study, we focused on miRNA mimics in order to develop therapies for idiopathic pulmonary fibrosis. We developed a single-stranded RNA, termed "miR-29b Psh-match," that has a unique structure to avoid problems associated with the therapeutic uses of miRNAs. A comparison of miR-29b Psh-match and double-stranded one, termed "miR-29b mimic" indicated that the single-stranded form was significantly effective towards fibrosis according to both in vivo and in vitro experiments. This novel form of miR-29b may become the foundation for developing an effective therapeutic drug for pulmonary fibrosis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0171957