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Chitosan-assisted differentiation of porcine adipose tissue-derived stem cells into glucose-responsive insulin-secreting clusters
The unique advantage of easy access and abundance make the adipose-derived stem cells (ADSCs) a promising system of multipotent cells for transplantation and regenerative medicine. Among the available sources, porcine ADSCs (pADSCs) deserve especial attention due to the close resemblance of human an...
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| Published in: | PloS one 2017-03, Vol.12 (3), p.e0172922-e0172922 |
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| container_end_page | e0172922 |
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| creator | Liu, Hui-Yu Chen, Chih-Chien Lin, Yuan-Yu Chen, Yu-Jen Liu, Bing-Hsien Wong, Shiu-Chung Wu, Cheng-Yu Chang, Yun-Tsui Chou, Han-Yi E Ding, Shih-Torng |
| description | The unique advantage of easy access and abundance make the adipose-derived stem cells (ADSCs) a promising system of multipotent cells for transplantation and regenerative medicine. Among the available sources, porcine ADSCs (pADSCs) deserve especial attention due to the close resemblance of human and porcine physiology, as well as for the upcoming availability of humanized porcine models. Here, we report on the isolation and conversion of pADSCs into glucose-responsive insulin-secreting cells. We used the stromal-vascular fraction of the dorsal subcutaneous adipose from 9-day-old male piglets to isolate pADSCs, and subjected the cells to an induction scheme for differentiation on chitosan-coated plates. This one-step procedure promoted differentiation of pADSCs into pancreatic islet-like clusters (PILC) that are characterized by the expression of a repertoire of pancreatic proteins, including pancreatic and duodenal homeobox (Pdx-1), insulin gene enhancer protein (ISL-1) and insulin. Upon glucose challenge, these PILC secreted high amounts of insulin in a dose-dependent manner. Our data also suggest that chitosan plays roles not only to enhance the differentiation potential of pADSCs, but also to increase the glucose responsiveness of PILCs. Our novel approach is, therefore, of great potential for transplantation-based amelioration of type 1 diabetes. |
| doi_str_mv | 10.1371/journal.pone.0172922 |
| format | article |
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Among the available sources, porcine ADSCs (pADSCs) deserve especial attention due to the close resemblance of human and porcine physiology, as well as for the upcoming availability of humanized porcine models. Here, we report on the isolation and conversion of pADSCs into glucose-responsive insulin-secreting cells. We used the stromal-vascular fraction of the dorsal subcutaneous adipose from 9-day-old male piglets to isolate pADSCs, and subjected the cells to an induction scheme for differentiation on chitosan-coated plates. This one-step procedure promoted differentiation of pADSCs into pancreatic islet-like clusters (PILC) that are characterized by the expression of a repertoire of pancreatic proteins, including pancreatic and duodenal homeobox (Pdx-1), insulin gene enhancer protein (ISL-1) and insulin. Upon glucose challenge, these PILC secreted high amounts of insulin in a dose-dependent manner. Our data also suggest that chitosan plays roles not only to enhance the differentiation potential of pADSCs, but also to increase the glucose responsiveness of PILCs. Our novel approach is, therefore, of great potential for transplantation-based amelioration of type 1 diabetes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0172922</identifier><identifier>PMID: 28253305</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adipose tissue ; Adipose Tissue - cytology ; Adipose Tissue - drug effects ; Animal models ; Animal sciences ; Animals ; Biology and Life Sciences ; Biomedical materials ; Biotechnology ; Body fat ; Bone marrow ; Cell culture ; Cell Differentiation - drug effects ; Chitin ; Chitosan ; Chitosan - pharmacology ; Clusters ; Culture Media ; Diabetes ; Diabetes mellitus ; Diabetes mellitus (insulin dependent) ; Differentiation ; Dosage ; Enzyme-Linked Immunosorbent Assay ; Fibroblasts ; Glucose ; Growth ; Growth factors ; Hogs ; Homeobox ; Innovations ; Insulin ; Insulin - metabolism ; Insulin Secretion ; Insurance claims ; Medicine ; Medicine and Health Sciences ; Metabolism ; Pancreas ; Physical Sciences ; Properties ; Proteins ; Regenerative medicine ; Research and Analysis Methods ; Rodents ; Stem cell transplantation ; Stem cells ; Stem Cells - cytology ; Stem Cells - drug effects ; Subcutaneous Fat - cytology ; Swine ; Tissue engineering ; Transplantation ; Zoology</subject><ispartof>PloS one, 2017-03, Vol.12 (3), p.e0172922-e0172922</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Liu et al 2017 Liu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-7ac5e27ac5bb821428a93522244cc89135f726e6ff0f25cad980068f02680ed63</citedby><cites>FETCH-LOGICAL-c725t-7ac5e27ac5bb821428a93522244cc89135f726e6ff0f25cad980068f02680ed63</cites><orcidid>0000-0002-9866-1776</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1873690879/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1873690879?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25752,27923,27924,37011,37012,44589,53790,53792,74997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28253305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kerkis, Irina</contributor><creatorcontrib>Liu, Hui-Yu</creatorcontrib><creatorcontrib>Chen, Chih-Chien</creatorcontrib><creatorcontrib>Lin, Yuan-Yu</creatorcontrib><creatorcontrib>Chen, Yu-Jen</creatorcontrib><creatorcontrib>Liu, Bing-Hsien</creatorcontrib><creatorcontrib>Wong, Shiu-Chung</creatorcontrib><creatorcontrib>Wu, Cheng-Yu</creatorcontrib><creatorcontrib>Chang, Yun-Tsui</creatorcontrib><creatorcontrib>Chou, Han-Yi E</creatorcontrib><creatorcontrib>Ding, Shih-Torng</creatorcontrib><title>Chitosan-assisted differentiation of porcine adipose tissue-derived stem cells into glucose-responsive insulin-secreting clusters</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The unique advantage of easy access and abundance make the adipose-derived stem cells (ADSCs) a promising system of multipotent cells for transplantation and regenerative medicine. Among the available sources, porcine ADSCs (pADSCs) deserve especial attention due to the close resemblance of human and porcine physiology, as well as for the upcoming availability of humanized porcine models. Here, we report on the isolation and conversion of pADSCs into glucose-responsive insulin-secreting cells. We used the stromal-vascular fraction of the dorsal subcutaneous adipose from 9-day-old male piglets to isolate pADSCs, and subjected the cells to an induction scheme for differentiation on chitosan-coated plates. This one-step procedure promoted differentiation of pADSCs into pancreatic islet-like clusters (PILC) that are characterized by the expression of a repertoire of pancreatic proteins, including pancreatic and duodenal homeobox (Pdx-1), insulin gene enhancer protein (ISL-1) and insulin. Upon glucose challenge, these PILC secreted high amounts of insulin in a dose-dependent manner. Our data also suggest that chitosan plays roles not only to enhance the differentiation potential of pADSCs, but also to increase the glucose responsiveness of PILCs. Our novel approach is, therefore, of great potential for transplantation-based amelioration of type 1 diabetes.</description><subject>Adipose tissue</subject><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - drug effects</subject><subject>Animal models</subject><subject>Animal sciences</subject><subject>Animals</subject><subject>Biology and Life Sciences</subject><subject>Biomedical materials</subject><subject>Biotechnology</subject><subject>Body fat</subject><subject>Bone marrow</subject><subject>Cell culture</subject><subject>Cell Differentiation - drug effects</subject><subject>Chitin</subject><subject>Chitosan</subject><subject>Chitosan - pharmacology</subject><subject>Clusters</subject><subject>Culture Media</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes mellitus (insulin dependent)</subject><subject>Differentiation</subject><subject>Dosage</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fibroblasts</subject><subject>Glucose</subject><subject>Growth</subject><subject>Growth factors</subject><subject>Hogs</subject><subject>Homeobox</subject><subject>Innovations</subject><subject>Insulin</subject><subject>Insulin - metabolism</subject><subject>Insulin Secretion</subject><subject>Insurance claims</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Metabolism</subject><subject>Pancreas</subject><subject>Physical Sciences</subject><subject>Properties</subject><subject>Proteins</subject><subject>Regenerative medicine</subject><subject>Research and Analysis Methods</subject><subject>Rodents</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Subcutaneous Fat - cytology</subject><subject>Swine</subject><subject>Tissue engineering</subject><subject>Transplantation</subject><subject>Zoology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tq3DAQhk1padK0b1BaQ6G0F97q4IN0UwhLDwuBQE-3QitLXi1aa6ORQ3vZN6-cdcK65CIYbDH6_t-jGU2WvcRogWmDP2z9EHrpFnvf6wXCDeGEPMpOMaekqAmij4_WJ9kzgC1CFWV1_TQ7IYxUlKLqNPu73NjoQfaFBLAQdZu31hgddB-tjNb3uTf53gdle53L1u496DxagEEXrQ72OimSbJcr7Rzkto8-79ygElYEDSk7SEyKw-BsX4BWQUfbd7lyQ9IFeJ49MdKBfjF9z7Kfnz_9WH4tLi6_rJbnF4VqSBWLRqpKk_G9XjOCS8IkpxUhpCyVYhzTyjSk1rUxyJBKyZYzhGpmEKkZ0m1Nz7LXB9-98yCm6oHArKE1R6zhiVgdiNbLrdgHu5Phj_DSipuAD52QIVrltOBrihFvjZFrWbYNYliplhBKq1KSulHJ6-P0t2G9061K5QzSzUznO73diM5fi9QXymiVDN5NBsFfDRqi2FkYayx77YebvBtGStzUD0HLssSco4S--Q-9vxAT1cl0Vtsbn1JUo6k4LxltSs4rkqjFPVR6Wr2zKt1KY1N8Jng_EyQm6t-xkwOAWH3_9nD28tecfXvEbrR0cQPeDePthTlYHkAVPEDQ5q4fGIlxqG6rIcahEtNQJdmr417eiW6niP4D6Fce7A</recordid><startdate>20170302</startdate><enddate>20170302</enddate><creator>Liu, Hui-Yu</creator><creator>Chen, Chih-Chien</creator><creator>Lin, Yuan-Yu</creator><creator>Chen, Yu-Jen</creator><creator>Liu, Bing-Hsien</creator><creator>Wong, Shiu-Chung</creator><creator>Wu, Cheng-Yu</creator><creator>Chang, Yun-Tsui</creator><creator>Chou, Han-Yi E</creator><creator>Ding, Shih-Torng</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-9866-1776</orcidid></search><sort><creationdate>20170302</creationdate><title>Chitosan-assisted differentiation of porcine adipose tissue-derived stem cells into glucose-responsive insulin-secreting clusters</title><author>Liu, Hui-Yu ; Chen, Chih-Chien ; Lin, Yuan-Yu ; Chen, Yu-Jen ; Liu, Bing-Hsien ; Wong, Shiu-Chung ; Wu, Cheng-Yu ; Chang, Yun-Tsui ; Chou, Han-Yi E ; Ding, Shih-Torng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-7ac5e27ac5bb821428a93522244cc89135f726e6ff0f25cad980068f02680ed63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adipose tissue</topic><topic>Adipose Tissue - 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Among the available sources, porcine ADSCs (pADSCs) deserve especial attention due to the close resemblance of human and porcine physiology, as well as for the upcoming availability of humanized porcine models. Here, we report on the isolation and conversion of pADSCs into glucose-responsive insulin-secreting cells. We used the stromal-vascular fraction of the dorsal subcutaneous adipose from 9-day-old male piglets to isolate pADSCs, and subjected the cells to an induction scheme for differentiation on chitosan-coated plates. This one-step procedure promoted differentiation of pADSCs into pancreatic islet-like clusters (PILC) that are characterized by the expression of a repertoire of pancreatic proteins, including pancreatic and duodenal homeobox (Pdx-1), insulin gene enhancer protein (ISL-1) and insulin. Upon glucose challenge, these PILC secreted high amounts of insulin in a dose-dependent manner. Our data also suggest that chitosan plays roles not only to enhance the differentiation potential of pADSCs, but also to increase the glucose responsiveness of PILCs. Our novel approach is, therefore, of great potential for transplantation-based amelioration of type 1 diabetes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28253305</pmid><doi>10.1371/journal.pone.0172922</doi><tpages>e0172922</tpages><orcidid>https://orcid.org/0000-0002-9866-1776</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-03, Vol.12 (3), p.e0172922-e0172922 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1873690879 |
| source | PubMed (Medline); Publicly Available Content Database (Proquest) (PQ_SDU_P3) |
| subjects | Adipose tissue Adipose Tissue - cytology Adipose Tissue - drug effects Animal models Animal sciences Animals Biology and Life Sciences Biomedical materials Biotechnology Body fat Bone marrow Cell culture Cell Differentiation - drug effects Chitin Chitosan Chitosan - pharmacology Clusters Culture Media Diabetes Diabetes mellitus Diabetes mellitus (insulin dependent) Differentiation Dosage Enzyme-Linked Immunosorbent Assay Fibroblasts Glucose Growth Growth factors Hogs Homeobox Innovations Insulin Insulin - metabolism Insulin Secretion Insurance claims Medicine Medicine and Health Sciences Metabolism Pancreas Physical Sciences Properties Proteins Regenerative medicine Research and Analysis Methods Rodents Stem cell transplantation Stem cells Stem Cells - cytology Stem Cells - drug effects Subcutaneous Fat - cytology Swine Tissue engineering Transplantation Zoology |
| title | Chitosan-assisted differentiation of porcine adipose tissue-derived stem cells into glucose-responsive insulin-secreting clusters |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T17%3A44%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Chitosan-assisted%20differentiation%20of%20porcine%20adipose%20tissue-derived%20stem%20cells%20into%20glucose-responsive%20insulin-secreting%20clusters&rft.jtitle=PloS%20one&rft.au=Liu,%20Hui-Yu&rft.date=2017-03-02&rft.volume=12&rft.issue=3&rft.spage=e0172922&rft.epage=e0172922&rft.pages=e0172922-e0172922&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0172922&rft_dat=%3Cgale_plos_%3EA483749952%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c725t-7ac5e27ac5bb821428a93522244cc89135f726e6ff0f25cad980068f02680ed63%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1873690879&rft_id=info:pmid/28253305&rft_galeid=A483749952&rfr_iscdi=true |