Cytokines and microbicidal molecules regulated by IL-32 in THP-1-derived human macrophages infected with New World Leishmania species

Interleukin-32 (IL-32) is expressed in lesions of patients with American Tegumentary Leishmaniasis (ATL), but its precise role in the disease remains unknown. In the present study, silencing and overexpression of IL-32 was performed in THP-1-derived macrophages infected with Leishmania (Viannia) bra...

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Published in:PLoS neglected tropical diseases 2017-02, Vol.11 (2), p.e0005413-e0005413
Main Authors: Dos Santos, Jéssica Cristina, Heinhuis, Bas, Gomes, Rodrigo Saar, Damen, Michelle S M A, Real, Fernando, Mortara, Renato A, Keating, Samuel T, Dinarello, Charles A, Joosten, Leo A B, Ribeiro-Dias, Fátima
Format: Article
Language:English
Subjects:
Antimicrobial Cationic Peptides - metabolism
beta-Defensins - metabolism
Biology and Life Sciences
Care and treatment
Cell Line
Cytokines
Cytokines - metabolism
Development and progression
Funding
Gene Expression
Gene Knockdown Techniques
Genetic aspects
Health aspects
Humans
Infections
Infectious diseases
Interleukins
Interleukins - metabolism
Internal medicine
Leishmania braziliensis - immunology
Leishmania mexicana - immunology
Leishmaniasis
Life Sciences
Macrophages - immunology
Macrophages - parasitology
Medical research
Medicine
Medicine and Health Sciences
Nitric oxide
Nitric Oxide - metabolism
Parasites
Parasitic diseases
Research and Analysis Methods
Studies
Tropical diseases
Tumor necrosis factor-TNF
Vector-borne diseases
Viral infections
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cited_by cdi_FETCH-LOGICAL-c658t-66ff62e915af2a14262ae091576db19aa3d62c872de119b7111c32c51fcca3d93
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creator Dos Santos, Jéssica Cristina
Heinhuis, Bas
Gomes, Rodrigo Saar
Damen, Michelle S M A
Real, Fernando
Mortara, Renato A
Keating, Samuel T
Dinarello, Charles A
Joosten, Leo A B
Ribeiro-Dias, Fátima
description Interleukin-32 (IL-32) is expressed in lesions of patients with American Tegumentary Leishmaniasis (ATL), but its precise role in the disease remains unknown. In the present study, silencing and overexpression of IL-32 was performed in THP-1-derived macrophages infected with Leishmania (Viannia) braziliensis or L. (Leishmania) amazonensis to investigate the role of IL-32 in infection. We report that Leishmania species induces IL-32γ, and show that intracellular IL-32γ protein production is dependent on endogenous TNFα. Silencing or overexpression of IL-32 demonstrated that this cytokine is closely related to TNFα and IL-8. Remarkably, the infection index was augmented in the absence of IL-32 and decreased in cells overexpressing this cytokine. Mechanistically, these effects can be explained by nitric oxide cathelicidin and β-defensin 2 production regulated by IL-32. Thus, endogenous IL-32 is a crucial cytokine involved in the host defense against Leishmania parasites.
doi_str_mv 10.1371/journal.pntd.0005413
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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: species. PLoS Negl Trop Dis 11(2): e0005413. doi:10.1371/journal.pntd.0005413</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>2017 dos Santos et al 2017 dos Santos et al</rights><rights>2017 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: species. 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identifier ISSN: 1935-2735
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issn 1935-2735
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language eng
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subjects Antimicrobial Cationic Peptides - metabolism
beta-Defensins - metabolism
Biology and Life Sciences
Care and treatment
Cell Line
Cytokines
Cytokines - metabolism
Development and progression
Funding
Gene Expression
Gene Knockdown Techniques
Genetic aspects
Health aspects
Humans
Infections
Infectious diseases
Interleukins
Interleukins - metabolism
Internal medicine
Leishmania braziliensis - immunology
Leishmania mexicana - immunology
Leishmaniasis
Life Sciences
Macrophages - immunology
Macrophages - parasitology
Medical research
Medicine
Medicine and Health Sciences
Nitric oxide
Nitric Oxide - metabolism
Parasites
Parasitic diseases
Research and Analysis Methods
Studies
Tropical diseases
Tumor necrosis factor-TNF
Vector-borne diseases
Viral infections
title Cytokines and microbicidal molecules regulated by IL-32 in THP-1-derived human macrophages infected with New World Leishmania species
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T08%3A41%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cytokines%20and%20microbicidal%20molecules%20regulated%20by%20IL-32%20in%20THP-1-derived%20human%20macrophages%20infected%20with%20New%20World%20Leishmania%20species&rft.jtitle=PLoS%20neglected%20tropical%20diseases&rft.au=Dos%20Santos,%20J%C3%A9ssica%20Cristina&rft.date=2017-02-27&rft.volume=11&rft.issue=2&rft.spage=e0005413&rft.epage=e0005413&rft.pages=e0005413-e0005413&rft.issn=1935-2735&rft.eissn=1935-2735&rft_id=info:doi/10.1371/journal.pntd.0005413&rft_dat=%3Cgale_plos_%3EA493820768%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c658t-66ff62e915af2a14262ae091576db19aa3d62c872de119b7111c32c51fcca3d93%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1878076103&rft_id=info:pmid/28241012&rft_galeid=A493820768&rfr_iscdi=true