Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver

Ubiquitin is a crucial post-translational modification regulating numerous cellular processes, but its role in metabolic disease is not well characterized. In this study, we identified the in vivo ubiquitin-modified proteome in rat liver and determined changes in this ubiquitome under acute insulin...

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Bibliographic Details
Published in:PloS one 2017-03, Vol.12 (3), p.e0174431-e0174431
Main Authors: Nagarajan, Shilpa R, Brandon, Amanda E, McKenna, Jessie A, Shtein, Harrison C, Nguyen, Thinh Q, Suryana, Eurwin, Poronnik, Philip, Cooney, Gregory J, Saunders, Darren N, Hoy, Andrew J
Format: Article
Language:English
Subjects:
Analysis
Animals
Biology and Life Sciences
Carbon - metabolism
Citric Acid Cycle - drug effects
Diet
Diet - methods
Dietary Fats - administration & dosage
Fatty acids
Fatty Acids - metabolism
Gluconeogenesis
Gluconeogenesis - drug effects
Glucose
Glycolysis
Glycolysis - drug effects
Health aspects
In vivo methods and tests
Insulin
Insulin - administration & dosage
Insulin resistance
Insulin Resistance - physiology
Kinases
Lipid Metabolism - drug effects
Liver
Liver - drug effects
Liver research
Male
Medicine and Health Sciences
Metabolic Networks and Pathways - drug effects
Metabolic pathways
Metabolism
Post-translation
Protein Processing, Post-Translational - drug effects
Proteins
Proteome - drug effects
Proteomes
Proteomics
Rats
Rats, Wistar
Rodents
Stimulation
Sucrose
Sucrose - administration & dosage
Sugar
Tricarboxylic acid cycle
Ubiquitin
Ubiquitin - metabolism
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Summary:Ubiquitin is a crucial post-translational modification regulating numerous cellular processes, but its role in metabolic disease is not well characterized. In this study, we identified the in vivo ubiquitin-modified proteome in rat liver and determined changes in this ubiquitome under acute insulin stimulation and high-fat and sucrose diet-induced insulin resistance. We identified 1267 ubiquitinated proteins in rat liver across diet and insulin-stimulated conditions, with 882 proteins common to all conditions. KEGG pathway analysis of these proteins identified enrichment of metabolic pathways, TCA cycle, glycolysis/gluconeogenesis, fatty acid metabolism, and carbon metabolism, with similar pathways altered by diet and insulin resistance. Thus, the rat liver ubiquitome is sensitive to diet and insulin stimulation and this is perturbed in insulin resistance.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0174431