Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver

Ubiquitin is a crucial post-translational modification regulating numerous cellular processes, but its role in metabolic disease is not well characterized. In this study, we identified the in vivo ubiquitin-modified proteome in rat liver and determined changes in this ubiquitome under acute insulin...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2017-03, Vol.12 (3), p.e0174431-e0174431
Main Authors: Nagarajan, Shilpa R, Brandon, Amanda E, McKenna, Jessie A, Shtein, Harrison C, Nguyen, Thinh Q, Suryana, Eurwin, Poronnik, Philip, Cooney, Gregory J, Saunders, Darren N, Hoy, Andrew J
Format: Article
Language:English
Subjects:
Analysis
Animals
Biology and Life Sciences
Carbon - metabolism
Citric Acid Cycle - drug effects
Diet
Diet - methods
Dietary Fats - administration & dosage
Fatty acids
Fatty Acids - metabolism
Gluconeogenesis
Gluconeogenesis - drug effects
Glucose
Glycolysis
Glycolysis - drug effects
Health aspects
In vivo methods and tests
Insulin
Insulin - administration & dosage
Insulin resistance
Insulin Resistance - physiology
Kinases
Lipid Metabolism - drug effects
Liver
Liver - drug effects
Liver research
Male
Medicine and Health Sciences
Metabolic Networks and Pathways - drug effects
Metabolic pathways
Metabolism
Post-translation
Protein Processing, Post-Translational - drug effects
Proteins
Proteome - drug effects
Proteomes
Proteomics
Rats
Rats, Wistar
Rodents
Stimulation
Sucrose
Sucrose - administration & dosage
Sugar
Tricarboxylic acid cycle
Ubiquitin
Ubiquitin - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c725t-e9896cc49ff8c238d9d5697ca4db8c557369f77063aa0d25c1868c3eafa95163
cites cdi_FETCH-LOGICAL-c725t-e9896cc49ff8c238d9d5697ca4db8c557369f77063aa0d25c1868c3eafa95163
container_end_page e0174431
container_issue 3
container_start_page e0174431
container_title PloS one
container_volume 12
creator Nagarajan, Shilpa R
Brandon, Amanda E
McKenna, Jessie A
Shtein, Harrison C
Nguyen, Thinh Q
Suryana, Eurwin
Poronnik, Philip
Cooney, Gregory J
Saunders, Darren N
Hoy, Andrew J
description Ubiquitin is a crucial post-translational modification regulating numerous cellular processes, but its role in metabolic disease is not well characterized. In this study, we identified the in vivo ubiquitin-modified proteome in rat liver and determined changes in this ubiquitome under acute insulin stimulation and high-fat and sucrose diet-induced insulin resistance. We identified 1267 ubiquitinated proteins in rat liver across diet and insulin-stimulated conditions, with 882 proteins common to all conditions. KEGG pathway analysis of these proteins identified enrichment of metabolic pathways, TCA cycle, glycolysis/gluconeogenesis, fatty acid metabolism, and carbon metabolism, with similar pathways altered by diet and insulin resistance. Thus, the rat liver ubiquitome is sensitive to diet and insulin stimulation and this is perturbed in insulin resistance.
doi_str_mv 10.1371/journal.pone.0174431
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1879964538</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A486629836</galeid><doaj_id>oai_doaj_org_article_826c149944114ab5a491cc52c94e644a</doaj_id><sourcerecordid>A486629836</sourcerecordid><originalsourceid>FETCH-LOGICAL-c725t-e9896cc49ff8c238d9d5697ca4db8c557369f77063aa0d25c1868c3eafa95163</originalsourceid><addsrcrecordid>eNqNk11rFDEUhgdRbK3-A9EBQfRi1nxNJrkplOLHQqGixduQTTK7KZlkm2SK_nuz7rTsSC9KLhJOnvfNyUlOVb2GYAFxBz9dhzF66Rbb4M0CwI4QDJ9Ux5Bj1FAE8NOD9VH1IqVrAFrMKH1eHSGGEQeAHVfflz6Nzvpael1ra3JjvR6V0bXaSL82qS57eWPqcWVvRputb4agbW8LsY0hmzCYOvR1lLl29tbEl9WzXrpkXk3zSXX15fPV-bfm4vLr8vzsolEdanNjOONUKcL7nimEmea6pbxTkugVU23bYcr7rgMUSwk0ahVklClsZC95Cyk-qd7ubbcuJDHVIgnIOs4pKfcsxHJP6CCvxTbaQcY_Ikgr_gVCXAsZs1XOCIaogoRzQiAkctVKwqFSLVKcGEqILF6n02njajBaGZ-jdDPT-Y63G7EOt6LFFCHcFYMPk0EMN6NJWQw2KeOc9CaMu7wZ4x1H9FEoIB0CgBT03X_ow4WYqLUsd7W-DyVFtTMVZ6T8B8QZ3hV08QBVhjaDVeWP9bbEZ4KPM0Fhsvmd13JMSSx__ng8e_lrzr4_YDdGurxJwY3ZBp_mINmDKoaUounv3wMCsWuRu2qIXYuIqUWK7M3hW96L7noC_wWmHAo6</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1879964538</pqid></control><display><type>article</type><title>Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Nagarajan, Shilpa R ; Brandon, Amanda E ; McKenna, Jessie A ; Shtein, Harrison C ; Nguyen, Thinh Q ; Suryana, Eurwin ; Poronnik, Philip ; Cooney, Gregory J ; Saunders, Darren N ; Hoy, Andrew J</creator><contributor>Vinciguerra, Manlio</contributor><creatorcontrib>Nagarajan, Shilpa R ; Brandon, Amanda E ; McKenna, Jessie A ; Shtein, Harrison C ; Nguyen, Thinh Q ; Suryana, Eurwin ; Poronnik, Philip ; Cooney, Gregory J ; Saunders, Darren N ; Hoy, Andrew J ; Vinciguerra, Manlio</creatorcontrib><description>Ubiquitin is a crucial post-translational modification regulating numerous cellular processes, but its role in metabolic disease is not well characterized. In this study, we identified the in vivo ubiquitin-modified proteome in rat liver and determined changes in this ubiquitome under acute insulin stimulation and high-fat and sucrose diet-induced insulin resistance. We identified 1267 ubiquitinated proteins in rat liver across diet and insulin-stimulated conditions, with 882 proteins common to all conditions. KEGG pathway analysis of these proteins identified enrichment of metabolic pathways, TCA cycle, glycolysis/gluconeogenesis, fatty acid metabolism, and carbon metabolism, with similar pathways altered by diet and insulin resistance. Thus, the rat liver ubiquitome is sensitive to diet and insulin stimulation and this is perturbed in insulin resistance.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0174431</identifier><identifier>PMID: 28329008</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Biology and Life Sciences ; Carbon - metabolism ; Citric Acid Cycle - drug effects ; Diet ; Diet - methods ; Dietary Fats - administration &amp; dosage ; Fatty acids ; Fatty Acids - metabolism ; Gluconeogenesis ; Gluconeogenesis - drug effects ; Glucose ; Glycolysis ; Glycolysis - drug effects ; Health aspects ; In vivo methods and tests ; Insulin ; Insulin - administration &amp; dosage ; Insulin resistance ; Insulin Resistance - physiology ; Kinases ; Lipid Metabolism - drug effects ; Liver ; Liver - drug effects ; Liver research ; Male ; Medicine and Health Sciences ; Metabolic Networks and Pathways - drug effects ; Metabolic pathways ; Metabolism ; Post-translation ; Protein Processing, Post-Translational - drug effects ; Proteins ; Proteome - drug effects ; Proteomes ; Proteomics ; Rats ; Rats, Wistar ; Rodents ; Stimulation ; Sucrose ; Sucrose - administration &amp; dosage ; Sugar ; Tricarboxylic acid cycle ; Ubiquitin ; Ubiquitin - metabolism</subject><ispartof>PloS one, 2017-03, Vol.12 (3), p.e0174431-e0174431</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Nagarajan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Nagarajan et al 2017 Nagarajan et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-e9896cc49ff8c238d9d5697ca4db8c557369f77063aa0d25c1868c3eafa95163</citedby><cites>FETCH-LOGICAL-c725t-e9896cc49ff8c238d9d5697ca4db8c557369f77063aa0d25c1868c3eafa95163</cites><orcidid>0000-0003-3922-1137</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1879964538/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1879964538?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28329008$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Vinciguerra, Manlio</contributor><creatorcontrib>Nagarajan, Shilpa R</creatorcontrib><creatorcontrib>Brandon, Amanda E</creatorcontrib><creatorcontrib>McKenna, Jessie A</creatorcontrib><creatorcontrib>Shtein, Harrison C</creatorcontrib><creatorcontrib>Nguyen, Thinh Q</creatorcontrib><creatorcontrib>Suryana, Eurwin</creatorcontrib><creatorcontrib>Poronnik, Philip</creatorcontrib><creatorcontrib>Cooney, Gregory J</creatorcontrib><creatorcontrib>Saunders, Darren N</creatorcontrib><creatorcontrib>Hoy, Andrew J</creatorcontrib><title>Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Ubiquitin is a crucial post-translational modification regulating numerous cellular processes, but its role in metabolic disease is not well characterized. In this study, we identified the in vivo ubiquitin-modified proteome in rat liver and determined changes in this ubiquitome under acute insulin stimulation and high-fat and sucrose diet-induced insulin resistance. We identified 1267 ubiquitinated proteins in rat liver across diet and insulin-stimulated conditions, with 882 proteins common to all conditions. KEGG pathway analysis of these proteins identified enrichment of metabolic pathways, TCA cycle, glycolysis/gluconeogenesis, fatty acid metabolism, and carbon metabolism, with similar pathways altered by diet and insulin resistance. Thus, the rat liver ubiquitome is sensitive to diet and insulin stimulation and this is perturbed in insulin resistance.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biology and Life Sciences</subject><subject>Carbon - metabolism</subject><subject>Citric Acid Cycle - drug effects</subject><subject>Diet</subject><subject>Diet - methods</subject><subject>Dietary Fats - administration &amp; dosage</subject><subject>Fatty acids</subject><subject>Fatty Acids - metabolism</subject><subject>Gluconeogenesis</subject><subject>Gluconeogenesis - drug effects</subject><subject>Glucose</subject><subject>Glycolysis</subject><subject>Glycolysis - drug effects</subject><subject>Health aspects</subject><subject>In vivo methods and tests</subject><subject>Insulin</subject><subject>Insulin - administration &amp; dosage</subject><subject>Insulin resistance</subject><subject>Insulin Resistance - physiology</subject><subject>Kinases</subject><subject>Lipid Metabolism - drug effects</subject><subject>Liver</subject><subject>Liver - drug effects</subject><subject>Liver research</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Metabolic Networks and Pathways - drug effects</subject><subject>Metabolic pathways</subject><subject>Metabolism</subject><subject>Post-translation</subject><subject>Protein Processing, Post-Translational - drug effects</subject><subject>Proteins</subject><subject>Proteome - drug effects</subject><subject>Proteomes</subject><subject>Proteomics</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Rodents</subject><subject>Stimulation</subject><subject>Sucrose</subject><subject>Sucrose - administration &amp; dosage</subject><subject>Sugar</subject><subject>Tricarboxylic acid cycle</subject><subject>Ubiquitin</subject><subject>Ubiquitin - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11rFDEUhgdRbK3-A9EBQfRi1nxNJrkplOLHQqGixduQTTK7KZlkm2SK_nuz7rTsSC9KLhJOnvfNyUlOVb2GYAFxBz9dhzF66Rbb4M0CwI4QDJ9Ux5Bj1FAE8NOD9VH1IqVrAFrMKH1eHSGGEQeAHVfflz6Nzvpael1ra3JjvR6V0bXaSL82qS57eWPqcWVvRputb4agbW8LsY0hmzCYOvR1lLl29tbEl9WzXrpkXk3zSXX15fPV-bfm4vLr8vzsolEdanNjOONUKcL7nimEmea6pbxTkugVU23bYcr7rgMUSwk0ahVklClsZC95Cyk-qd7ubbcuJDHVIgnIOs4pKfcsxHJP6CCvxTbaQcY_Ikgr_gVCXAsZs1XOCIaogoRzQiAkctVKwqFSLVKcGEqILF6n02njajBaGZ-jdDPT-Y63G7EOt6LFFCHcFYMPk0EMN6NJWQw2KeOc9CaMu7wZ4x1H9FEoIB0CgBT03X_ow4WYqLUsd7W-DyVFtTMVZ6T8B8QZ3hV08QBVhjaDVeWP9bbEZ4KPM0Fhsvmd13JMSSx__ng8e_lrzr4_YDdGurxJwY3ZBp_mINmDKoaUounv3wMCsWuRu2qIXYuIqUWK7M3hW96L7noC_wWmHAo6</recordid><startdate>20170322</startdate><enddate>20170322</enddate><creator>Nagarajan, Shilpa R</creator><creator>Brandon, Amanda E</creator><creator>McKenna, Jessie A</creator><creator>Shtein, Harrison C</creator><creator>Nguyen, Thinh Q</creator><creator>Suryana, Eurwin</creator><creator>Poronnik, Philip</creator><creator>Cooney, Gregory J</creator><creator>Saunders, Darren N</creator><creator>Hoy, Andrew J</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-3922-1137</orcidid></search><sort><creationdate>20170322</creationdate><title>Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver</title><author>Nagarajan, Shilpa R ; Brandon, Amanda E ; McKenna, Jessie A ; Shtein, Harrison C ; Nguyen, Thinh Q ; Suryana, Eurwin ; Poronnik, Philip ; Cooney, Gregory J ; Saunders, Darren N ; Hoy, Andrew J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-e9896cc49ff8c238d9d5697ca4db8c557369f77063aa0d25c1868c3eafa95163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Biology and Life Sciences</topic><topic>Carbon - metabolism</topic><topic>Citric Acid Cycle - drug effects</topic><topic>Diet</topic><topic>Diet - methods</topic><topic>Dietary Fats - administration &amp; dosage</topic><topic>Fatty acids</topic><topic>Fatty Acids - metabolism</topic><topic>Gluconeogenesis</topic><topic>Gluconeogenesis - drug effects</topic><topic>Glucose</topic><topic>Glycolysis</topic><topic>Glycolysis - drug effects</topic><topic>Health aspects</topic><topic>In vivo methods and tests</topic><topic>Insulin</topic><topic>Insulin - administration &amp; dosage</topic><topic>Insulin resistance</topic><topic>Insulin Resistance - physiology</topic><topic>Kinases</topic><topic>Lipid Metabolism - drug effects</topic><topic>Liver</topic><topic>Liver - drug effects</topic><topic>Liver research</topic><topic>Male</topic><topic>Medicine and Health Sciences</topic><topic>Metabolic Networks and Pathways - drug effects</topic><topic>Metabolic pathways</topic><topic>Metabolism</topic><topic>Post-translation</topic><topic>Protein Processing, Post-Translational - drug effects</topic><topic>Proteins</topic><topic>Proteome - drug effects</topic><topic>Proteomes</topic><topic>Proteomics</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Rodents</topic><topic>Stimulation</topic><topic>Sucrose</topic><topic>Sucrose - administration &amp; dosage</topic><topic>Sugar</topic><topic>Tricarboxylic acid cycle</topic><topic>Ubiquitin</topic><topic>Ubiquitin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nagarajan, Shilpa R</creatorcontrib><creatorcontrib>Brandon, Amanda E</creatorcontrib><creatorcontrib>McKenna, Jessie A</creatorcontrib><creatorcontrib>Shtein, Harrison C</creatorcontrib><creatorcontrib>Nguyen, Thinh Q</creatorcontrib><creatorcontrib>Suryana, Eurwin</creatorcontrib><creatorcontrib>Poronnik, Philip</creatorcontrib><creatorcontrib>Cooney, Gregory J</creatorcontrib><creatorcontrib>Saunders, Darren N</creatorcontrib><creatorcontrib>Hoy, Andrew J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest_Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>https://resources.nclive.org/materials</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nagarajan, Shilpa R</au><au>Brandon, Amanda E</au><au>McKenna, Jessie A</au><au>Shtein, Harrison C</au><au>Nguyen, Thinh Q</au><au>Suryana, Eurwin</au><au>Poronnik, Philip</au><au>Cooney, Gregory J</au><au>Saunders, Darren N</au><au>Hoy, Andrew J</au><au>Vinciguerra, Manlio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-03-22</date><risdate>2017</risdate><volume>12</volume><issue>3</issue><spage>e0174431</spage><epage>e0174431</epage><pages>e0174431-e0174431</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Ubiquitin is a crucial post-translational modification regulating numerous cellular processes, but its role in metabolic disease is not well characterized. In this study, we identified the in vivo ubiquitin-modified proteome in rat liver and determined changes in this ubiquitome under acute insulin stimulation and high-fat and sucrose diet-induced insulin resistance. We identified 1267 ubiquitinated proteins in rat liver across diet and insulin-stimulated conditions, with 882 proteins common to all conditions. KEGG pathway analysis of these proteins identified enrichment of metabolic pathways, TCA cycle, glycolysis/gluconeogenesis, fatty acid metabolism, and carbon metabolism, with similar pathways altered by diet and insulin resistance. Thus, the rat liver ubiquitome is sensitive to diet and insulin stimulation and this is perturbed in insulin resistance.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28329008</pmid><doi>10.1371/journal.pone.0174431</doi><tpages>e0174431</tpages><orcidid>https://orcid.org/0000-0003-3922-1137</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2017-03, Vol.12 (3), p.e0174431-e0174431
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1879964538
source Publicly Available Content Database; PubMed Central
subjects Analysis
Animals
Biology and Life Sciences
Carbon - metabolism
Citric Acid Cycle - drug effects
Diet
Diet - methods
Dietary Fats - administration & dosage
Fatty acids
Fatty Acids - metabolism
Gluconeogenesis
Gluconeogenesis - drug effects
Glucose
Glycolysis
Glycolysis - drug effects
Health aspects
In vivo methods and tests
Insulin
Insulin - administration & dosage
Insulin resistance
Insulin Resistance - physiology
Kinases
Lipid Metabolism - drug effects
Liver
Liver - drug effects
Liver research
Male
Medicine and Health Sciences
Metabolic Networks and Pathways - drug effects
Metabolic pathways
Metabolism
Post-translation
Protein Processing, Post-Translational - drug effects
Proteins
Proteome - drug effects
Proteomes
Proteomics
Rats
Rats, Wistar
Rodents
Stimulation
Sucrose
Sucrose - administration & dosage
Sugar
Tricarboxylic acid cycle
Ubiquitin
Ubiquitin - metabolism
title Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T13%3A17%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Insulin%20and%20diet-induced%20changes%20in%20the%20ubiquitin-modified%20proteome%20of%20rat%20liver&rft.jtitle=PloS%20one&rft.au=Nagarajan,%20Shilpa%20R&rft.date=2017-03-22&rft.volume=12&rft.issue=3&rft.spage=e0174431&rft.epage=e0174431&rft.pages=e0174431-e0174431&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0174431&rft_dat=%3Cgale_plos_%3EA486629836%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c725t-e9896cc49ff8c238d9d5697ca4db8c557369f77063aa0d25c1868c3eafa95163%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1879964538&rft_id=info:pmid/28329008&rft_galeid=A486629836&rfr_iscdi=true