Sympathetic nervous system activity and anti-lipolytic response to iv-glucose load in subcutaneous adipose tissue of obese and obese type 2 diabetic subjects

• Published in: PloS one 2017-03, Vol.12 (3), p.e0173803-e0173803
• Main Authors: Schumann, Uwe, Jenkinson, Christopher P, Alt, Andreas, Zügel, Martina, Steinacker, Jürgen M, Flechtner-Mors, Marion
• Format: Article
• Language: English
• Subjects: Adipocytes; Adipose tissue; Adrenergic Agonists - administration & dosage; Adrenergic Agonists - pharmacology; Adult; Biology and Life Sciences; Blood; Blood flow; Blood Glucose - analysis; Body fat; Body mass; Diabetes; Diabetes mellitus; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - physiopathology; Diagnosis; Ethanol; Experiments; Female; Flow; Glucose; Glucose - administration & dosage; Glycerol; Glycerol - blood; Humans; Inhibition; Insulin; Insulin - blood; Insulin resistance; Legal medicine; Lipolysis; Lipolysis - drug effects; Medicine; Medicine and Health Sciences; Metabolism; Middle Aged; Musculoskeletal system; Nervous system; Norepinephrine; Norepinephrine - administration & dosage; Norepinephrine - pharmacology; Obesity; Obesity - blood; Obesity - complications; Obesity - physiopathology; Octopamine - administration & dosage; Octopamine - analogs & derivatives; Octopamine - pharmacology; Physical sciences; Physiological aspects; Regulation; Rehabilitation; Risk factors; Stimulation; Subcutaneous Fat - blood supply; Subcutaneous Fat - drug effects; Subcutaneous Fat - innervation; Subcutaneous Fat - physiopathology; Sympathetic nervous system; Sympathetic Nervous System - drug effects; Sympathetic Nervous System - physiopathology; Type 2 diabetes; Young Adult
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0173803

The study aim was to investigate the effect of endogenous insulin release on lipolysis in subcutaneous adipose tissue after adrenergic stimulation in obese subjects diagnosed with type 2 diabetes (T2D). In 14 obese female T2D subjects, or 14 obese non-T2D controls, glycerol concentration was measured in response to the α1,2,ß-agonist norepinephrine, the α1-agonist norfenefrine and the ß2-agonist terbutaline (each 10-4 M), using the microdialysis technique. After 60 minutes of stimulation, an intravenous glucose load (0.5 g/kg lean body mass) was given. Local blood flow was monitored by means of the ethanol technique. Norepinephrine and norfenefrine induced a four and three fold rise in glycerol dialysate concentration (p