Isolation and characterization of the TaSnRK2.10 gene and its association with agronomic traits in wheat (Triticum aestivum L.)

• Published in: PloS one 2017-03, Vol.12 (3), p.e0174425-e0174425
• Main Authors: Zhang, Zhao-Gui, Lv, Guang-de, Li, Bing, Wang, Jia-Jia, Zhao, Yan, Kong, Fan-Mei, Guo, Ying, Li, Si-Shen
• Format: Article
• Language: English
• Subjects: Abiotic stress; Agricultural production; Agronomy; Alleles; Amino acids; Association analysis; Biology; Biology and Life Sciences; Bread; Carbohydrates; Chromosome Mapping; Chromosomes; Chromosomes, Plant - genetics; Cloning; Cloning, Molecular; Crops, Agricultural - genetics; Crops, Agricultural - growth & development; DNA, Plant - genetics; DNA, Plant - isolation & purification; Gene expression; Genes; Genes, Plant; Genetic aspects; Genetic Association Studies; Genetics; Grain; Haplotypes; Inbreeding; Kinases; Laboratories; Markers; Metabolism; Nutrient utilization; Phenotype; Physiological aspects; Plant genetics; Plant Proteins - genetics; Population; Promoter Regions, Genetic; Protein kinase; Protein kinases; Protein Kinases - genetics; Proteins; Research and Analysis Methods; Signal transduction; Signaling; Sucrose; Sugar; Triticum - genetics; Triticum - growth & development; Triticum aestivum; Wheat; Winter wheat
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0174425

Sucrose non-fermenting 1-related protein kinases (SnRKs) comprise a major family of signaling genes in plants and are associated with metabolic regulation, nutrient utilization and stress responses. This gene family has been proposed to be involved in sucrose signaling. In the present study, we cloned three copies of the TaSnRK2.10 gene from bread wheat on chromosomes 4A, 4B and 4D. The coding sequence (CDS) is 1086 bp in length and encodes a protein of 361 amino acids that exhibits functional domains shared with SnRK2s. Based on the haplotypes of TaSnRK2.10-4A (Hap-4A-H and Hap-4A-L), a cleaved amplified polymorphic sequence (CAPS) marker designated TaSnRK2.10-4A-CAPS was developed and mapped between the markers D-1092101 and D-100014232 using a set of recombinant inbred lines (RILs). The TaSnRK2.10-4B alleles (Hap-4B-G and Hap-4B-A) were transformed into allele-specific PCR (AS-PCR) markers TaSnRK2.10-4B-AS1 and TaSnRK2.10-4B-AS2, which were located between the markers D-1281577 and S-1862758. No diversity was found for TaSnRK2.10-4D. An association analysis using a natural population consisting of 128 winter wheat varieties in multiple environments showed that the thousand grain weight (TGW) and spike length (SL) of Hap-4A-H were significantly higher than those of Hap-4A-L, but pant height (PH) was significantly lower.