A novel synaptic junction preparation for the identification and characterization of cleft proteins

• Published in: PloS one 2017-03, Vol.12 (3), p.e0174895-e0174895
• Main Authors: Burch, Amelia, Tao-Cheng, Jung-Hwa, Dosemeci, Ayse
• Format: Article
• Language: English
• Subjects: Abundance; Adhesion; Adhesive strength; Alternative splicing; Analysis; Anatomy; Animals; Antibodies; Axon guidance; Axonogenesis; Biochemistry; Biology and Life Sciences; Blotting, Western; Brain; Carbon dioxide; Cell adhesion; Cell adhesion & migration; Cell Adhesion - physiology; Cell adhesion molecules; Centrifugation; Centrifuging; Classification; Compartments; Deglycosylation; Dendritic spines; Detergents; Dilution; Disks Large Homolog 4 Protein; Dopamine; Dopamine receptors; Electron microscopy; Enrichment; EphA4 protein; Forebrain; Fractionation; Hippocampus; Ice; Identification methods; Immunoglobulins; Intracellular Signaling Peptides and Proteins - metabolism; Laboratories; Localization; Long-term potentiation; Lysis; Markers; Medicine and Health Sciences; Membrane proteins; Membrane Proteins - metabolism; Membranes; Methods; Microscopy, Immunoelectron; Nervous system; Neurobiology; Neurological disorders; Neurosciences; Nitrogen; Ontogeny; Pellets; Phospholipase A2; Phospholipases A2 - metabolism; Plasmas (physics); Proteinase inhibitors; Proteins; Rats; Rats, Sprague-Dawley; Receptor, Metabotropic Glutamate 5 - metabolism; Research and Analysis Methods; Ribonuclease; Rodents; Spine; Stroke; Sucrose; Sugar; Synapses; Synapses - metabolism; Synapses - ultrastructure; Synaptic Membranes - metabolism; Synaptic Membranes - ultrastructure; Tagging; Topographic mapping; Venom
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0174895

Identification of synaptic cleft components has been hampered by the lack of a suitable preparation enriched in synaptic junctions devoid of adjoining peripheral membranes. Prior strategies for the isolation of synaptic junctions, relying on detergents for the removal of peripheral membranes, resulted in substantial loss of membranes lining the cleft. Here, a novel, detergent-free method is described for the preparation of a synaptic junction (SJ) fraction, using phospholipase A2. Limited digestion of synaptic plasma membrane (SPM) fraction with phospholipase A2 followed by centrifugation over a sucrose cushion results in selective removal of membranes peripheral to the cleft while junctional membranes remain relatively intact as observed by electron microscopy. Enrichment in synaptic junctional structures and loss of membranes peripheral to the junctional area are further verified by demonstrating enrichment in PSD-95 and loss in mGluR5, respectively. The SJ fraction is enriched in neuroligins and neurexins, in agreement with immuno-electron microscopy data showing their selective localization to the junctional area. Among additional cell adhesion molecules tested, N-cadherin and specific isoforms of the SynCAM and SALM families also show marked enrichment in the SJ fraction, suggesting preferential localization at the synaptic cleft while others show little enrichment or decrease, suggesting that they are not restricted to or concentrated at the synaptic cleft. Treatment of the SJ fraction with glycosidases results in electrophoretic mobility shifts of all cell adhesion molecules tested, indicating glycosylation at the synaptic cleft. Biochemical and ultrastructural data presented indicate that the novel synaptic junction preparation can be used as a predictive tool for the identification and characterization of the components of the synaptic cleft.