ANKRD54 preferentially selects Bruton's Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library

Bruton's Tyrosine Kinase (BTK) is a cytoplasmic protein tyrosine kinase with a fundamental role in B-lymphocyte development and activation. The nucleocytoplasmic shuttling of BTK is specifically modulated by the Ankyrin Repeat Domain 54 (ANKRD54) protein and the interaction is known to be exclu...

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Bibliographic Details
Published in:PloS one 2017, Vol.12 (4), p.e0174909-e0174909
Main Authors: Gustafsson, Manuela O, Mohammad, Dara K, Ylösmäki, Erkko, Choi, Hyunseok, Shrestha, Subhash, Wang, Qing, Nore, Beston F, Saksela, Kalle, Smith, C I Edvard
Format: Article
Language:English
Subjects:
Agammaglobulinaemia Tyrosine Kinase
Animals
Ankyrins
B-Lymphocytes - cytology
Binding sites
Biochemistry
Biology
Biology and Life Sciences
Bruton's tyrosine kinase
Cell activation
Cell Line, Tumor
Cercopithecus aethiops
COS Cells
Functional analysis
HEK293 Cells
Homology
Humans
Kinases
Laboratories
Libraries
Ligands
Lymphocytes B
Medical screening
Medicin och hälsovetenskap
Medicine
Medicine and Health Sciences
Mutation
Mutation - genetics
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Peptide Library
Phages
Protein Binding - genetics
Protein-tyrosine kinase
Protein-Tyrosine Kinases - genetics
Protein-Tyrosine Kinases - metabolism
Proteins
Research and Analysis Methods
Signal Transduction
src Homology Domains - genetics
src Homology Domains - physiology
Src protein
Tissues
Tyrosine
Virology
Western blotting
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Summary:Bruton's Tyrosine Kinase (BTK) is a cytoplasmic protein tyrosine kinase with a fundamental role in B-lymphocyte development and activation. The nucleocytoplasmic shuttling of BTK is specifically modulated by the Ankyrin Repeat Domain 54 (ANKRD54) protein and the interaction is known to be exclusively SH3-dependent. To identify the spectrum of the ANKRD54 SH3-interactome, we applied phage-display screening of a library containing all the 296 human SH3 domains. The BTK-SH3 domain was the prime interactor. Quantitative western blotting analysis demonstrated the accuracy of the screening procedure. Revealing the spectrum and specificity of ANKRD54-interactome is a critical step toward functional analysis in cells and tissues.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0174909