Turing mechanism underlying a branching model for lung morphogenesis

• Published in: PloS one 2017-04, Vol.12 (4), p.e0174946-e0174946
• Main Authors: Xu, Hui, Sun, Mingzhu, Zhao, Xin
• Format: Article
• Language: English
• Subjects: Alveoli; Animal models; Animals; Appendix; Bifurcations; Biochemistry; Biological models (mathematics); Biology; Biology and Life Sciences; Branching; Branching processes; Cell culture; Cell differentiation; Collagen; Collagenase; Computer Simulation; Cornea; Cysts; Decay; Differentiation; Diffusion; Dynamical systems; Dynamics; Epidemics; Epithelial cells; Genetic control; Genetics; Health aspects; Humans; Information systems; Inhibition; Inhibitors; Kinetics; Limb buds; Lung; Lung - growth & development; Lungs; Lymphocytes B; Mammals; Mammals - growth & development; Mathematical models; Mesenchyme; Models, Biological; Molecular biology; Morphogenesis; Organogenesis; Organs; Pattern formation; Physical Sciences; Physics; Plotting; Reaction kinetics; Research and Analysis Methods; Robotics; Shells; Simulation; Skin; Splitting; Stimulation; Terrestrial environments; Tips; Trajectories; Trajectory analysis; Vegetation
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0174946

The mammalian lung develops through branching morphogenesis. Two primary forms of branching, which occur in order, in the lung have been identified: tip bifurcation and side branching. However, the mechanisms of lung branching morphogenesis remain to be explored. In our previous study, a biological mechanism was presented for lung branching pattern formation through a branching model. Here, we provide a mathematical mechanism underlying the branching patterns. By decoupling the branching model, we demonstrated the existence of Turing instability. We performed Turing instability analysis to reveal the mathematical mechanism of the branching patterns. Our simulation results show that the Turing patterns underlying the branching patterns are spot patterns that exhibit high local morphogen concentration. The high local morphogen concentration induces the growth of branching. Furthermore, we found that the sparse spot patterns underlie the tip bifurcation patterns, while the dense spot patterns underlies the side branching patterns. The dispersion relation analysis shows that the Turing wavelength affects the branching structure. As the wavelength decreases, the spot patterns change from sparse to dense, the rate of tip bifurcation decreases and side branching eventually occurs instead. In the process of transformation, there may exists hybrid branching that mixes tip bifurcation and side branching. Since experimental studies have reported that branching mode switching from side branching to tip bifurcation in the lung is under genetic control, our simulation results suggest that genes control the switch of the branching mode by regulating the Turing wavelength. Our results provide a novel insight into and understanding of the formation of branching patterns in the lung and other biological systems.