Transplantation of induced mesenchymal stem cells for treating chronic renal insufficiency

• Published in: PloS one 2017-04, Vol.12 (4), p.e0176273-e0176273
• Main Authors: Pan, Xing-Hua, Zhou, Jing, Yao, Xiang, Shu, Jun, Liu, Ju-Fen, Yang, Jian-Yong, Pang, Rong-Qing, Ruan, Guang-Ping
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Biology and Life Sciences; Blood Urea Nitrogen; Bone marrow; Care and treatment; Cell growth; Cells, Cultured; Chronic kidney failure; Creatinine - blood; Disease Models, Animal; Engineering; Fibrosis; Glomerular Filtration Rate; Growth factors; Hospitals; Hypoxia; Immunohistochemistry; Immunology; Immunotherapy; In vitro methods and tests; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - transplantation; Kidney - pathology; Kidney diseases; Laboratories; Leukocytes, Mononuclear - cytology; Medicine and Health Sciences; Mesenchymal Stem Cell Transplantation; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mesenchyme; Nanog Homeobox Protein - genetics; Nanog Homeobox Protein - metabolism; Octamer Transcription Factor-3 - genetics; Octamer Transcription Factor-3 - metabolism; Patient outcomes; Phenotype; Phenotypes; Rabbits; Renal Insufficiency, Chronic - etiology; Renal Insufficiency, Chronic - pathology; Renal Insufficiency, Chronic - therapy; Research and Analysis Methods; Reservoirs; Stem cell transplantation; Stem cells; Studies; Technology transfer; Transforming Growth Factor beta1 - metabolism; Transplantation; Transplantation, Autologous; Transplants & implants; Tumor necrosis factor-TNF; Ureteral Obstruction - complications; Ureteral Obstruction - pathology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0176273

Discovering a new cell transplantation approach for treating chronic renal insufficiency is a goal of many nephrologists. In vitro-cultured peripheral blood mononuclear cells (PBMCs) were reprogrammed into induced mesenchymal stem cells (iMSCs) by using natural inducing agents made in our laboratory. The stem cell phenotype of the iMSCs was then identified. Unilateral ureteral obstruction (UUO) was used to create an animal model of chronic renal insufficiency characterized by renal interstitial fibrosis. The induced and non-induced PBMCs were transplanted, and the efficacy of iMSCs in treating chronic renal insufficiency was evaluated using a variety of methods. The ultimate goal was to explore the effects of iMSC transplantation on the treatment of chronic renal insufficiency, with the aim of providing a new therapeutic modality for this disease.