Nano-sized Al2O3 reduces acute toxic effects of thiacloprid on the non-biting midge Chironomus riparius

• Published in: PloS one 2017-05, Vol.12 (5), p.e0176356-e0176356
• Main Authors: Lorenz, Carla S, Wicht, Anna-J, Guluzada, Leyla, Luo, Leilei, Jäger, Leonie, Crone, Barbara, Karst, Uwe, Triebskorn, Rita, Liang, Yucang, Anwander, Reiner, Haderlein, Stefan B, Huhn, Carolin, Köhler, Heinz-R
• Format: Article
• Language: English
• Subjects: Acute toxicity; Adsorption; Aluminum; Aluminum oxide; Aluminum Oxide - administration & dosage; Analytical chemistry; Animals; Binary mixtures; Bioavailability; Biology and Life Sciences; Biota; Chemical analysis; Chironomidae - drug effects; Chromatography; Ecology; Engineering and Technology; Environmental science; Evolution; Exposure; Inorganic chemistry; Insecticides; Insecticides - administration & dosage; Insecticides - pharmacology; Larva - drug effects; Larvae; Medicine and Health Sciences; Mineralogy; Mortality; Nanomaterials; Nanoparticles; Nanoparticles - administration & dosage; Neonicotinoids; People and Places; Pesticides; Physical Sciences; Physiology; Pollutants; Pyridines - administration & dosage; Pyridines - pharmacology; Sediments; Sorption; Studies; Thiazines - administration & dosage; Thiazines - pharmacology; Toxicity testing
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0176356

This study focuses on interactions between nanoparticles and a pesticide. The aim was to investigate how nano-sized aluminum oxide (410 nm) can alter the toxic effects of thiacloprid, even if no sorption between particles and the insecticide takes place. Thus, our study investigated a rather unexplored interaction. We conducted our research with larvae of Chironomus riparius and used thiacloprid as test substance as its toxicity to C. riparius is well described. The used nano-Al2O3 particles where chosen due to their suitable properties. For testing the acute effects of the interaction, we exposed larvae to thiacloprid (0.5, 1.0, 2.0, and 5.0 μg/L) and nano-Al2O3 (300 and 1000 mg/L), either solely or in binary mixtures. While thiacloprid resulted in elevated mortality, nano-Al2O3 solely did not exert any effects. Moreover, we observed an aggregation of nano-Al2O3 within the lumen of the intestinal tract of the larvae. Further results showed a significantly reduced mortality of fourth instar larvae when they were exposed to mixtures of nanoparticles and the pesticide, compared to thiacloprid alone. With increasing nano-Al2O3 concentration, this effect became gradually stronger. Additionally, chemical analyses of internal thiacloprid concentrations implicate reduced uptake of thiacloprid in animals exposed to mixtures. However, as larvae exposed to thiacloprid concentrations > 0.5 μg/L showed severe convulsions, independent of the presence or concentration of nano-Al2O3, we assume that nano-Al2O3 leads to a delay of mortality and does not entirely prevent it. As sorption measurements on pristine or defecated nano-Al2O3 did not reveal any sorptive interaction with thiacloprid, we can exclude sorption-based reduction of thiacloprid bioavailability as a mechanism behind our results. Even though we used test substances which might not co-occur in the environment in the tested concentrations, our study gives evidence for an interaction besides adsorption, which is important to generally understand how nanoparticles might affect biota.